现代妇产科进展2013，Vol.22Issue(8)：626-629,4.
转化生长因子βⅡ受体在小鼠卵泡早期发育中的表达
Expression of transforming growth factors-β Ⅱ receptor during early ovarian folliculogenesis in mice
摘要
Abstract
Objective:We provided a theoretical basis for the regulatory mechanism of follicular development by investigating the dynamic expressions of TβR Ⅱ proteins and mRNA during early folliculogenesis in mouse ovaries.Method:Ovaries were collected from the healthy neonatal female mice at the age of 0,1,2,5,7,and 11 days.The expressions of TβR Ⅱ proteins and mRNA were detected and evaluated by immunohistochemistry and Real-time polymerase chain reaction respectively.Results:TβR Ⅱ proteins were expressed in the cytoplasm of oocytes within ovaries in different age stages.The highest intensity of TβR Ⅱ staining was present in germ cell cysts.Primordial follicles exhibited moderate staining,and the staining of TβR Ⅱ increased during the transition to in primary and secondary follicles.For granulosa cells,TβR Ⅱ expression was found to be primarily in the primary follicles and increased with follicular development.There was no significant difference in TβR Ⅱ mRNA level among the ovary tissues of 0,1 and 11 days(P＞0.05).However,the ovarian TβR Ⅱ mRNA level during these periods was 2-fold higher than these of 2,5 and 7 days(P＜0.05).Conclusion:The expression of TβR Ⅱ proteins and mRNA in mice ovary suggests that the TGF-β signaling pathway may play an important role during early folliculogenesis.关键词
转化生长因子βⅡ受体(TβRⅡ)/卵泡发育/免疫组织化学/实时荧光定量聚合酶链反应/小鼠Key words
Transforming growth factors-βⅡ receptor/Folliculogenesis/Immunohistochemisty/Real-time polymerase chain reaction/Mice分类
医药卫生引用本文复制引用
王硕,杨书红,马兰芳,罗爱月,李亚..转化生长因子βⅡ受体在小鼠卵泡早期发育中的表达[J].现代妇产科进展,2013,22(8):626-629,4.基金项目
卫生部公益性行业科研专项基金(No:200802159) （No:200802159）
教育部高等学校博士点科研基金(No:200804870009) （No:200804870009）
国家自然科学基金(No:30973148) （No:30973148）
