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碳量子点的制备及与牛血清蛋白的相互作用

吕华 单晓辉 陈娇 蒋瑀涵 陈建秋 严拯宇

新型炭材料2013,Vol.28Issue(4):307-312,6.
新型炭材料2013,Vol.28Issue(4):307-312,6.

碳量子点的制备及与牛血清蛋白的相互作用

Preparation of carbon quantum dots in wood charcoal and their interaction with bovine serum albumin

吕华 1单晓辉 1陈娇 1蒋瑀涵 1陈建秋 1严拯宇1

作者信息

  • 1. 中国药科大学分析化学教研室,江苏南京210009
  • 折叠

摘要

Abstract

Carbon quantum dots (CQDs) are a new fluorescent material with stable fluorescence,low toxicity and many other superior properties that make their applications in biology possible.CQDs in wood charcoal were created by a 5N HNO3 solution using three different methods,reflux,microwave and ultrasound methods.Factors that determine the fluorescence quantum yield were examined and preparation conditions were optimized.Results indicated that the fluorescence quantum yield of CQDs prepared by the reflux method was highest among the three methods.The CQDs prepared by this method were modified by poly (ethylene glycol)2000 to increase the fluorescent lifetime and the quantum yield.The reaction of the modified CQDs with bovine serum albumin was investigated by ultraviolet absorption spectrometry and fluorescence spectrometry,which revealed a static quenching behavior.

关键词

碳量子点/制备/荧光/牛血清白蛋白

Key words

Carbon quantum dots/ Synthesis/ Fluorescence/ Bovine serum albumin

分类

通用工业技术

引用本文复制引用

吕华,单晓辉,陈娇,蒋瑀涵,陈建秋,严拯宇..碳量子点的制备及与牛血清蛋白的相互作用[J].新型炭材料,2013,28(4):307-312,6.

基金项目

国家自然科学基金(30873197) (30873197)

大学生创新基金(201210316099).National Nature Science Foundation (30873197) (201210316099)

Fundamental Research Funds for the Central Universities (201210316099). (201210316099)

新型炭材料

OA北大核心CSCDCSTPCDSCI

1007-8827

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