应用化学2013，Vol.30Issue(9)：1030-1035,6.DOI:10.3724/SP.J.1095.2013.20496
无溶剂体系中固定化脂肪酶Candida sp.99-125催化合成油酸低碳醇酯
Immobilized-Lipase (Candida sp.99-125)-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems
摘要
Abstract
The esterification between oleic acid and alkyl alcohols in solvent-free systems were catalyzed by an immobilized lipase from Candida sp.99-125.The effects of several factors including enzyme concentration,temperature,molar ratio of oleic acid to alkyl alcohols,and structure of alcohols have been also investigated.The results indicated that the reactions catalyzed by lipase at 20 ℃ with the presence of 3 ％ (mass fraction)lipase,a 1 ∶ 1 molar ratio of oleic acid to alcohols,afforded products in high yields.Methanol has certain toxicity on the activity of the lipase.The enzyme showed high selectivity to primary alcohols and low selectivity to secondary and tertiary alcohols because of the sterical effect.It showed high activity to long chain fatty acids and low activity to polybasic acids and polyhydric alcohols with branched chain.The lipase showed no appreciable loss in activity after being continuously operated for 5 times.The enzymatic synthesis gave purer products,compared with the conventional chemical system.关键词
生物催化/固定化脂肪酶/酯化/油酸低碳醇酯/无溶剂体系Key words
enzymatic catalysis/ immobilized lipase/ esterification/ oleates/ solvent-free system分类
化学化工引用本文复制引用
仲蕙,方正,邹宝华,李昕,郭凯..无溶剂体系中固定化脂肪酶Candida sp.99-125催化合成油酸低碳醇酯[J].应用化学,2013,30(9):1030-1035,6.基金项目
国家重点基础研究发展计划资助项目(973计划,2012CB725204和2011CB710803) （973计划,2012CB725204和2011CB710803）
长江学者和创新团队发展计划资助项目(IRT1066) （IRT1066）
