应用化学2013，Vol.30Issue(9)：1096-1098,3.DOI:10.3724/SP.J.1095.2013.30012
流动注射-石英晶体微天平中血清蛋白对扁桃酸的手性识别
Chiral Recognition of Mandelic Acid with Bovine and Goat Serum Albumins by Flow Injection Analysis-Quartz Crystal Microbalance
摘要
Abstract
Interactions of mandelic acid (MA) and bovine/goat serum albumins (BSA/GSA) have been investigated by FIA-QCM and UV-Vis spectroscopy,respectively.Based on frequency shift of quartz crystal,surface concentrations of immobilized BSA and GSA were 4.9 × 10ˉ12 and 8.8 × 10ˉ12 mol/cm2,respectively.After injecting mandelic acid into FIA-QCM system,frequency of the quartz crystal decreases due to interactions between enantiomers and SA molecules and the shifts between SA and R-MA are greater than those with S-enantiomer,indicating that the interactions of SA molecules with R-MA are stronger than those with S-MA.The UV binding constants for SA with S-MA are smaller than those with R-enantiomer.Taking into account the discrimination factors(αQCM and αUv),GSA shows a better chiral recognition with mandelic acid than BSA.关键词
血清蛋白/扁桃酸/流动注射-石英晶体微天平/紫外光谱/识别因子Key words
serum albumin/ mandelic acid /flow injection analysis-quartz crystal microbalance/ UV-Vis spectroscopy/ discrimination factor分类
化学化工引用本文复制引用
洪涛,王秋云,范军,陈文静,郑盛润,章伟光..流动注射-石英晶体微天平中血清蛋白对扁桃酸的手性识别[J].应用化学,2013,30(9):1096-1098,3.基金项目
国家自然科学基金(21171059)和广东省科技计划项目(2010B050300003,2010B050300021,2011B010400023)资助 （21171059）
