诊断学理论与实践2013，Vol.12Issue(4)：414-418,5.DOI:10.3969/j.issn.1673-6087.2013.04.008
PI3K/mTOR双重抑制剂NVP-BEZ235对弥漫大B细胞株靶向作用的体外研究
The in vitro study of targeting activity of a novel dual PI3K/mTOR inhibitor NVP-BEZ235 on diffuse large B-cell lymphoma cell line
摘要
Abstract
Objective:To investigate the targeting activity mechanism of a novel dual PI3K/mTOR inhibitor NVP-BEZ235 on diffuse large B-cell lymphoma cell lines in vitro.Methods:CCK-8 assay was used to assess the influence of NVP-BEZ235 on cell proliferation.Flow cytometry was performed to examine the cell cycle and apoptosis.The targeted proteins,changes of up-and down-stream molecules in PI3K/AKT/mTOR signaling pathway,cell cycle proteins and apoptosis-related proteins were detected by Western blot.Result:Cell proliferation was inhibited in a dose-dependent manner,cell cycle was arrested in G0/G1 phase and cell apoptosis was induced.Western blot assay showed that NVP-BEZ235 could inhibit the activity of key molecule of PI3K/AKT/mTOR pathway,down-regulate cell cycle-related proteins,and up-regulate apoptosis associated proteins.Conclusions:The dual PI3K/mTOR inhibitor NVP-BEZ235 could inhibit the proliferation and induce the apoptosis of diffuse large B-cell lymphoma cell lines SUDHL-4 and DB in vitro.关键词
PI3K/mTOR途径/NVP-BEZ235/弥漫大B细胞株/凋亡/细胞周期Key words
PI3K/AKT/mTOR pathway/ NVP-BEZ235/ Diffuse large B-cell lymphoma cell lines/ Apoptosis/ Cell cycle分类
医药卫生引用本文复制引用
付婉彬,王文方,刘之茵,陈丽韵,郭沛,李军民,徐子真..PI3K/mTOR双重抑制剂NVP-BEZ235对弥漫大B细胞株靶向作用的体外研究[J].诊断学理论与实践,2013,12(4):414-418,5.基金项目
上海市科学技术委员会科研创新项目(11140901200) （11140901200）
