中国农业科学2013，Vol.46Issue(10)：2004-2013,10.DOI:10.3864/j.issn.0578-1752.2013.10.005
多叶苜蓿花药培养技术体系的建立
Construction of a Highly Efficient Technology System in Anther Culture of Mutlifoliate Alfalfa
摘要
Abstract
[Objective] This experiment was carried out to establish an efficient anther culture system of mutlifoliate alfalfa,and get regenerate plantlet.It will also provide basic materials for the research ofploidy,genomics and molecular biology of alfalfa.[Method] Firstly,Huaiyin alfalfa and a mutlifoliate alfalfa varieties named Grandeur were used as the parents to create a hybrid,and the anther of hybrid F1 was used as the explants.The ability of induction of callus after bud was studied by using the culturemedium of NB added with 2,4-D (0.5 mg.L1),NAA (0.3 mg·L-1) and KT (3.0 mg.L1).The appropriate medium was screened for the differentiation of anther callus of alfalfa by orthogonal design L16 (45).In addition,the rooting ability from buds was studied by adding different concentrations of NAA into 1/2 B5 and 1/2 MS.[Result] The highest induction rate of alfalfa callus was in 15 to 28 days after budding,and the maximum value was 82.79％.The culture medium that was good for the differentiation of the callus of alfalfa anther was made up ofB I with CH (200 mg·L1),2-IP (0.5 mg·L1),NAA (0.2 mg·L1) and KT (1.0 mg·L1),and the maximum regeneration rate was 54.05％.The best culture medium for adventitious bud was 1/2 B5 with NAA (0.3 mg·L1),and the maximum rooting rate was 88.46％.[Conclusion] An efficient anther culture system of mutlifoliate alfalfa was established successfully,and the regenerated plants were obtained.关键词
苜蓿/花药培养/培养基/芽的分化Key words
alfalfa/ anther culture/ medium/ buds differentiation引用本文复制引用
武自念,魏臻武,郑曦..多叶苜蓿花药培养技术体系的建立[J].中国农业科学,2013,46(10):2004-2013,10.基金项目
国家"863"(2008AA10Z149)、国家自然科学基金项目(30972136)、江苏省普通高校研究生科研创新计划资助项目(CXZZ11_0991) （2008AA10Z149）
