中国农业科学2013，Vol.46Issue(10)：2014-2021,8.DOI:10.3864/j.issn.0578-1752.2013.10.006
解淀粉芽孢杆菌Lx-11产脂肽类物质鉴定及表面活性素对水稻细菌性条斑病的防治作用
Identification of the Lipopeptides from Bacillus amyloliquefaciens Lx-11 and Biocontrol Efficacy of Surfactin Against Bacterial Leaf Streak
摘要
Abstract
[Objective] The objective of this study is to isolate and identify the lipopeptides produced by Bacillus amyloliquefaciens Lx-11 and their biocontrol efficacy against bacterial leaf streak (BLS).[Method] Three primers designed based on the known lipopeptide genes were used to amplify the corresponding genes from the genome of Lx-11 strain.Lipopeptides were identified by MALDI-TOF-MS techniques.[Result] The PCR products with three primer pairs were cloned and sequenced.The BLAST analysis showed that the sfp,fenB,ituA or bamA genes exist in the genome of B.amyloliquefaciens Lx-11.Three kinds of lipopeptides surfactin,bacillomycin D and fengycin were identified in the antimicrobial extract by the mass spectrum analysis.The antibacterial activity could be associated with surfactin-lipopeptides,and practically abolished in surfactin-deficient mutants.Lx-11 strain significantly reduced disease incidence of BLS.[Conclusion] The antibacterial activity of the surfactin-deficient mutant strain was practically abolished,which suggested that surfactin might play an important role in control of BLS.关键词
解淀粉芽孢杆菌/细菌性条斑病/脂肽类物质/生物防治Key words
Bacillus amyloliquefaciens/ bacterial leaf streak (BLS)/ lipopeptides/ biological control引用本文复制引用
张荣胜,王晓宇,罗楚平,刘永锋,刘邮洲,陈志谊..解淀粉芽孢杆菌Lx-11产脂肽类物质鉴定及表面活性素对水稻细菌性条斑病的防治作用[J].中国农业科学,2013,46(10):2014-2021,8.基金项目
国家公益性行业(农业)科研专项经费项目(201103002-3,201303015)、江苏省农业科技自主创新资金项目(CX(11)2045) （农业）
