中国农业科学2013，Vol.46Issue(10)：2022-2031,10.DOI:10.3864/j.issn.0578-1752.2013.10.007
小麦NBS-LRR类抗病基因类似片段分离和定位
Isolation and Mapping of NBS-LRR Resistance Gene Homology Sequences from Wheat
摘要
Abstract
[Objective] The objective of this study is to isolate NBS-LRR class resistance gene homology fragments from wheat using homology-based method,and to validate the relationships with wheat stripe rust resistance gene Yr26.[Method] Primers were designed based on the conserved domains of the cloned plant disease resistance genes,and the nested PCR was used.The fragments were localized by Chinese Spring (CS) deletion lines of chromosome 1B,F2∶3 populations derived from susceptible cultivar Avocet S and the lines 92R137 carrying Yr26 were used to evaluate the relationship between the isolated RGAs and Yr26.[Result] Four open-reading resistance gene analogues (RGAs) were obtained,i.e.,R105,R181,R326 and R405.The nucleotide sequences of the four RGAs were 369,589,528 and 618 bp.Homology research showed that the four fragments had typical conserved regions NBS-LRR,and the nucleotide identity of four fragments was from 24.35％ to 38.36％.Only R405 was mapped in the deletion bin C-1BL-0.6-0.32 with Chinese Spring deletion lines and was co-segregated with a F2 population of 196 plants.It was initially hypothesized that the R405 might be the candidate sequence of Yr26.[Conclusion] In this study,four resistance homology fragments were obtained in the near isogenic lines (NILs) Nan137.R405 was mapped in the Yr26 region and co-segregated with Yr26,which provides a good prerequisite for further study.关键词
小麦/同源片段/巢式PCR/Yr26/NBS-LRRKey words
common wheat/ resistance gene analogs/ nested PCR/ Yr26/ NBS-LRR引用本文复制引用
史静东,张小娟,黄丽丽,韩德俊,康振生..小麦NBS-LRR类抗病基因类似片段分离和定位[J].中国农业科学,2013,46(10):2022-2031,10.基金项目
国家转基因重大专项(2009ZX08009-051B)、国家高技术研究发展"863"计划(2012AA101503)、国家公益性行业(农业)计划项目(201203014) （2009ZX08009-051B）
