中国农业科学2013，Vol.46Issue(10)：2041-2049,9.DOI:10.3864/j.issn.0578-1752.2013.10.009
烟碱对B型和Q型烟粉虱取食行为的影响——基于EPG和液体饲囊技术体系
Effects of Nicotine on Feeding Behavior of Bemisia tabaci B and Q Biotypes Based on EPG and Liquid Diet Sac Technique
摘要
Abstract
[Objective] The effects of nicotine on the feeding behaviors of the Bemisia tabaci B and Q biotypes were investigated,with the aim of establishing a corresponding evaluating system based on the electrical penetration graph (EPG) and the liquid diet sac (LDS) technique.[Method] EPG method combined with a LDS was used to quantify the effect of the concentrations of nicotine on the feeding behaviors of the B.tabaci.[Result] Relative amplitudes and frequencies in EPG waveforms of the B.tabaci in 10％ sucrose solution,compared to those in 5％ and 15％ sucrose solutions,were analogous to the waveforms ofB.tabaci on plants.The durations of ingestion waveforms decreased with the increase of nicotine levels in 10％ sucrose solution.Salivation (El) and passive ingestion (E2) durations were reduced and the penetration duration was prolonged even at the low concentration (0.001％) of nicotine.The passive ingestion from LDS was completely inhibited at a higher concentration of nicotine (0.01％).Although the major EPG pathway variables between B.tabaci B and Q biotypes were similar,the duration of passive ingestion of Q biotype was significantly longer than that of the B biotype on the diet without nicotine or with lower nicotine concentration.[Conclusion] The valuable EPG variables for EPG+LDS system include the durations of C,E1 and E2 pattems.Ten percentsucrose solution in LDS was more suitable for recording the feeding behaviors of B.tabaci using EPG technique.The feeding behaviors orB.tabaci B and Q biotypes were significantly inhibited by nicotine,but Q biotype had higher nicotine adaptability than B biotype.关键词
烟粉虱/液体饲囊/刺吸电位技术(EPG)/烟碱/取食行为Key words
Bemisia tabaci/ liquid diet sac (LDS)/ electrical penetration graph (EPG)/ nicotine/ feeding behaviors引用本文复制引用
李晓敏,李静静,汤清波,闫凤鸣..烟碱对B型和Q型烟粉虱取食行为的影响——基于EPG和液体饲囊技术体系[J].中国农业科学,2013,46(10):2041-2049,9.基金项目
国家转基因新品种培育重大专项项目(2009ZX08012-007B) （2009ZX08012-007B）
