中国农业科学2013，Vol.46Issue(12)：2459-2470,12.DOI:10.3864/j.issn.0578-1752.2013.12.007
紫花苜蓿对蓟马危害的光合生理响应
Photosynthetic Physiology of Odontothrips Damaged Medicago sativa
摘要
Abstract
[Objective] Photosynthetic physiological response mechanism of alfalfa to thrips and compensational mechanism of thrip damage were studied.[Method] Thrip resistant clone R-1 and susceptible clone I-1 were used to investigate the changes of gas exchange parameter and kinetic parameter of chlorophyll fluorescence of differently damaged leaves of R-1 and I-1 at initial flower stage under continuous thrips damage.[Result]As the increase of leaf damage grade,the chlorophyll content and water use efficiency (WUE) increased,net photosynthetic rate (Pn) increased firstly,then decreased,while intracellular CO2 concentration (Ci),transpiration rate (Tr) and stomatal conductance (Gs) decreased.Light compensation point (Lcp),dark respiration rate (Rd),apparent quantum yield (AQY),light saturation point (Lsp),maximum net photosynthetic rate (Pnmax) increased firstly and then decreased in R-l,and for I-1,Lcp and Rd increased,AQY and Lsp decreased,Pnmax increased firstly and then decreased.Under the same damage level,Pn,AQY,Lsp and Pnmax of R-1 were higher than I-1,while Rd and Lcp were lower than I-1.As the increase of leaf damage grade,F0 of R-1 decreased firstly and then increased,photosynthetic efficiency of PSⅡ (ΦPSⅡ),non-photochemical quenching coefficient (NPQ),photochemical quenching coefficient (qP),potential activity of PSⅡ (Fv/Fo) and original light energy transformation efficiency of PSⅡ(Fv/Fm) increased firstly,then decreased,F0 of I-1 increased,while NPQ and qP increased firstly,then decreased,ΦPSⅡ,actual Fv/F0,and Fv/Fm decreased.Under the same damage grade,R-1 was found with lower F0 and higher ΦPSⅡ,qP,Fv/F0 and Fv/Fm,compared with I-1.Comparatively,gas exchange parameter,light response parameter and kinetic parameter of chlorophyll fluorescence fluctuations of R-1 were all less than that of I-1.[Conclusion] Alfalfa PSⅡ reaction center was injured due to thrip damage,which resulted in a decreased photosynthetic efficiency,indicating that R-1 was with a higher photosynthetic efficiency and a more effective photosynthetic compensation efficiency under minor thrip damage compared with I-1,as well as a relatively stronger resistance against thrip damage.关键词
苜蓿无性系/蓟马/受害级别/光合生理/抗虫性Key words
alfalfa clone/ thrip/ damage grade/ photosynthetic physiology/ insect resistance引用本文复制引用
寇江涛,师尚礼,胡桂馨,周万海,姚拓..紫花苜蓿对蓟马危害的光合生理响应[J].中国农业科学,2013,46(12):2459-2470,12.基金项目
国家现代牧草产业技术体系建设专项(CARA-35)、教育部高校博士学科点基金(20106202110003)、国家自然科学基金(31260579) （CARA-35）
