中国农业科学2013，Vol.46Issue(12)：2543-2549,7.DOI:10.3864/j.issn.0578-1752.2013.12.015
HMGCS1基因对猪肌肉生长发育的影响及miRNA-18a/b对HMGCS1基因的调控
Effects of HMGCS1 Gene on Skeletal Muscle Growth and Development and Regulated by miR-18a/b of Pig
摘要
Abstract
[Objective] The purpose of the present research was to explore whether HMGCS 1 impacts the skeletal muscle growth and development of pig and validate its target gene.[Method] In this study,the expression of HMGCSI in different tissues and the expression change during Longissimus dorsi muscle development (28 stages) was investigated by real-time qPCR.[Result] The result showed that the HMGCS1 gene was strongly expressed in lung of adult pig,and the expression level of HMGCS1 gene in embryonic period 33 d and 65 d were higher than those in other periods in Tongcheng pigs,and lower expression at postnatal 20 d,30 d,40 d and 60 d.The luciferase activity was lower in miR-18a/b minics than in the control.[Conclusion] The results of study indicated that HMGCS1 regulates skeletal muscle growth and development by miR-18a/b,and is a potential candidate target gene of meat production traits.关键词
猪/HMGCS1基因/miR-18a/b/骨骼肌/生长发育Key words
pig/ HMGCS1 gene/ miR-18a/b/ skeletal muscle/ growth and development引用本文复制引用
孙伟,唐中林,谭林,雷初朝,李奎..HMGCS1基因对猪肌肉生长发育的影响及miRNA-18a/b对HMGCS1基因的调控[J].中国农业科学,2013,46(12):2543-2549,7.基金项目
国家自然科学基金项目(31171192,31172182)、国家重大科学研究计划(2012CB124706-6) （31171192,31172182）
