中国农业科学2013，Vol.46Issue(12)：2576-2583,8.DOI:10.3864/j.issn.0578-1752.2013.12.019
复方甘草酸单胺可溶性粉对恩诺沙星联合LPS致鸡肝损伤的免疫调节
Immunoregulatory Effects of Compound Ammonium Glycyrrhizin Soluble Powder on Liver Injury Induced by Enrofloxacin and LPS in Chickens
摘要
Abstract
[Objective] The present study was aimed at the effects of compound ammonium glycyrrhizin soluble powder (CAG) on proinflammatory cytokines production and T-cell subsets deviation in peripheral blood in chicken liver injury induced by enrofloxacin and lipopolysaccharide crude extract(LPS).[Method] A total of 104 animals were allocated randomly into 5 groups named Ⅰ-Ⅴ:Ⅰ normal group,Ⅱ liver injury group(model group),Ⅲ prevention group,Ⅳ high dosage treatment group and Ⅴ low dosage treatment group.Group Ⅱ-Ⅳ were administered with enrofloxacin(100 mg·kg-1) once a day for three days and on the third day administered with LPS (4 mL·kg-1) at the same time.Group Ⅲ received prior administration of CAG at the dosage of 40 mg.L-1 (by ammonium glycyrrhizin,the same as follows) in drinking water for three days before establishment of liver injury model.Groups Ⅳ and Ⅴ received CAG at a dosage of 22.5 and 7.5 mg·kg-1 twice a day for three days after LPS treatment.Every eight chickens were sacrificed at 6,24 and 48 h following post-treatment with LPS and their blood samples were collected for detection.[Result] Compared with group Ⅰ,the group Ⅱ levels of serum ALT,AST,TNF-α,IL-1 and IL-6 were significantly higher at 6,24,48 h post-treatment with LPS and CD3(+),CD4(+) and CD8(+) T-lymphocytes were significantly lower at 6 and 24 h higher at 48 h post-treatment with LPS.When compared with group Ⅱ,there existed a significant fall in the levels of serum ALT,TNF-α,IL-6 at 6 h,AST at 6 h and 24 h and the number of CD3(+),CD4(+) and CD8(+) T-lymphocytes at 48 h in group Ⅲ.In group Ⅳ,the levels of ALT,AST,TNF-α,IL-6 at 6 h and 24 h,IL-1 and CD3(+),CD4(+) and CD8(+) T-lymphocytes at 48 h decreased significantly and CD3(+) T-lymphocytes at 6 h and 24 h,CD4(+) T-lymphocytes at 24 h increased obviously.In group Ⅴ,there was a significant fall in CD8(+) T-lymphocytes at 48 h and no obvious other differences were detected.The liver histopathological changes got a better improvement in group Ⅳ than others.[Conclusion] The novel liver injury induced by enrofloxacin and LPS effects might be related to TNF-α,IL-1 and IL-6 production and CD3(+),CD4(+) and CD8(+) T-cell deviation in peripheral blood.CAG prevents the liver injury through inhibiting TNF-α,IL-1 and IL-6 production and regulating the number of T-cell subsets in peripheral blood by bi-directional regulation and the efficacy might be related to dosage and treatment time.关键词
复方甘草酸单胺可溶性粉/LPS/恩诺沙星/肝损伤/细胞因子/T淋巴细胞亚群Key words
compound ammonium glycyrrhizin soluble powder/ LPS/ enrofloxacin/ liver injury/ cytokines/ T-cell subsets引用本文复制引用
郭凡溪,刘腾飞,耿智霞,蒋凡,余祖功..复方甘草酸单胺可溶性粉对恩诺沙星联合LPS致鸡肝损伤的免疫调节[J].中国农业科学,2013,46(12):2576-2583,8.基金项目
国家自然科学基金项目(31172282) （31172282）
