中国农业科学2013，Vol.46Issue(12)：2607-2614,8.DOI:10.3864/j.issn.0578-1752.2013.12.023
崇明水仙试管球脱毒技术
The Virus-Free Technology of Bulblets of Chongming Daffodil (Narcissus tazetta var.chinensis) in vitro
摘要
Abstract
[Objective] The objective of this experiment is to explore a simple-operate method to eradicate viruses of Chongming Daffodil (Narcissus tazetta var.chinensis),and detect the effect of virus-free technology.[Method] The treatments of DHT+thermotherapy and DHT+virazole were used to eliminate viruses in bulblets in vitro of Chongming Daffodil,and the effect of treatments were tested by indicator plant and RT-PCR.[Result]Thermotherapy treatment had effect on the growth and survival rate of bulblets in vitro.Thermotherapy with DHT treatment decreased the content of viruses,and when the concentration of DHT was less than 15 mg·L-1,there was less inhibitory effect on growth.Virazole at 30 mg·L-1+DHT 30 mg·L-1 had good inhibitory effect on NMaV,and there was a very low level of virus detected by RT-PCR.NDV and NCLV had higher sensitivity to two antiviral compounds,and could be mostly eradicated.[Conclusion] There was a negative effect on thermotherapy treatment.DHT with thermotherapy treatment could decrease the content of viruses detected by indicator plant.The content of NMaV in bulblets in vitro treated with 30 mg L-1DHT +30 mg L-1vrazole was reduced to a very low level,and the treatment had no impact on bulblet's survival.NDV-free and NCLV-free plants were obtained by the treatment of combination with 10 mg L1DHT and 10 mg L1virazole,this result was proved by RT-PCR detection.关键词
崇明水仙/病毒脱除/热处理/化学药剂/RT-PCR分子检测Key words
Chongming Daffodil(Narcissus tazetta var.chinensis)/ virus elimination/ thermotherapy/ antiviral compounds/ RT-PCR detection引用本文复制引用
杨柳燕,张永春,李玉秀,戴富明..崇明水仙试管球脱毒技术[J].中国农业科学,2013,46(12):2607-2614,8.基金项目
上海市科技兴农攻关项目(沪农科攻字2005第1-2号)、上海市科技兴农推广项目"崇明水仙良种繁育及其优质栽培技术示范推广" （沪农科攻字2005第1-2号）
