中国药房2013，Vol.24Issue(27)：2513-2515,3.DOI:10.6039/j.issn.1001-0408.2013.27.06
参附注射液预处理对心肌缺血再灌注损伤模型大鼠的保护作用研究
Protective Effects of Shenfu Injection Pretreatment on Myocardial Ischemia-reperfusion Injury Model Rats
谭冰 1熊毅2
作者信息
- 1. 重庆市中山医院药剂科,重庆400010
- 2. 重庆市沙坪坝区人民医院药剂科,重庆400030
- 折叠
摘要
Abstract
OBJECTIVE:To investigate the protective effects of Shenfu injection (SFI) on myocardial ischemia-reperfusion injury (MI/RI) model rats.METHODS:MI/RI model was established by opening the chest and ligating the anterior descending branches of the left coronary arteries (LAD) for 30 min,followed by 120 min reperfusion.Model rats were divided into normal control group (constant volume of normal saline),sham operation control group (constant volume of normal saline),model group(constant volume of normal saline) and SFI group(10 mg/kg).The activities of SOD and GSH-PX in the myocardial tissue and the activities of CK and LDH in the serum were all determined.Flow cytometry was employed to assay the expression of apoptosis related genes Bcl-2 and Bax in the blood plasma.RESULTS:Compared with sham operation group,the activities of SOD and GSH-PX were decreased significantly in model group,while the activities of CK and LDH were enhanced significantly; the content of MDA and the expression of Bax were increased significantly while the expression of Bcl-2 was decreased significantly(P＜001or P＜0.05).Compared with model group,the activities of SOD and GSH-PX and the expression of Bcl-2 were increased significantly in SFI group while the activities of CK and LDH,the content of MDA and the expression of Bax were decreased significantly(P＜0.01 or P＜0.05).CONCLUSIONS:SFI pretreatment have a protective effect on MFRI,which might be associated with anti-oxygen free radical,protecting muscular tissues and suppressing myocardial cells apoptosis.关键词
参附注射液/心肌缺血再灌注损伤/氧自由基/凋亡Key words
Shenfu injection/Myocardial ischemia-reperfusion injury/Oxygen free radical/Apoptosis分类
医药卫生引用本文复制引用
谭冰,熊毅..参附注射液预处理对心肌缺血再灌注损伤模型大鼠的保护作用研究[J].中国药房,2013,24(27):2513-2515,3.
