中国药房2013，Vol.24Issue(27)：2526-2528,3.DOI:10.6039/j.issn.1001-0408.2013.27.11
正交试验优选南山茶花中总黄酮的提取工艺
Optimization of Extraction Technology of Total Flavonoids from the Flowers of Camellia semiserrata by Orthogonal Test
摘要
Abstract
OBJECTIVE:To optimize the extraction technology for total flavonoids from the flowers of Camellia semiserrata.METHODS:The total flavonoids were extracted from the flowers with EtOH-H2O.The extraction technology was optimized by orthogonal test with enthanol concentration,extraction time,extraction temperature and ratio of material to liquid as factor using yield of total flavonoids as index.RESULTS:The optimal extraction technology was as follows:20-fold 85 ％ ethanol,extracting 2 times at 95 ℃ for 3 hours.By this technology,the yield of total flavonoids was 1.52％.CONCLUSIONS:Optimized technology was reasonable and feasible,which can be applied for the extraction of total flavonoids from the flowers of C.semiserrata.关键词
南山茶花/总黄酮/提取工艺/正交试验Key words
Camellia semiserrata/Total flavonoids/Extraction technology/Orthogonal test分类
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尚志春,王宝昌,张微微,彭倩,唐玲,王永奇,史丽颖..正交试验优选南山茶花中总黄酮的提取工艺[J].中国药房,2013,24(27):2526-2528,3.基金项目
国家自然科学基金资助项目(No.30772714) （No.30772714）
