中国药房2013，Vol.24Issue(27)：2535-2537,3.DOI:10.6039/j.issn.1001-0408.2013.27.14
正交试验优选黄蜀葵花中总黄酮的提取工艺
Optimization of the Extraction Process for Total Flavonoids from Abelmoschus manihot by Orthogonal Test
李春花 1刘雪丽 2郑文丽 3张华潭 3魏艳婷 3武朝阳3
作者信息
- 1. 河北医科大学中医学院中药药剂教研室,石家庄050091
- 2. 灵寿县医院,河北灵寿050500
- 3. 河北医科大学研究生学院,石家庄050017
- 折叠
摘要
Abstract
OBJECTIVE:To optimize the extraction process of total flavonoids of Abelmoschus manihot.METHODS:The acidification technology was optimized by orthogonal experiment with the content of quercetin as index using reflux time,reflux temperature,amount of acid fluid,ratio of acid fluid (ethanol ∶ 25％ hydrochloric acid) as factors.The extraction technology was optimized using ethanol concentration,ethanol consumption,extraction time and extraction times as factors.RESULTS:Optimized acidification technology was as follows:25-folds of ethanol-25％ hydrochloric acid (4∶1,V/V) as acid fluid,70 ℃ water bath for refluxing 0.5 h; optimized extraction technology was as follows:12-folds of 85％ ethanol,extracting 2 times,2.5 h each time.CONCLUSIONS:Optimized technology is reasonable and feasible,and can be used for the extraction of total flavonoids from A.manihot.关键词
正交试验/黄蜀葵花/总黄酮/槲皮素/提取工艺Key words
Orthogonal experiment/Abelmoschus manihot/Total flavonoids/Quercetin/Extraction technology分类
医药卫生引用本文复制引用
李春花,刘雪丽,郑文丽,张华潭,魏艳婷,武朝阳..正交试验优选黄蜀葵花中总黄酮的提取工艺[J].中国药房,2013,24(27):2535-2537,3.
