中国药房2013，Vol.24Issue(27)：2549-2551,3.DOI:10.6039/j.issn.1001-0408.2013.27.19
黄柏-苍术药对不同比例配伍中生物碱含量的变化
Content Change of Alkaloids in Different Compatibility Proportions of Phellodendron chinense and Atractylodes lancea
摘要
Abstract
OBJECTIVE:To explore the variation of alkaloids content in the compatibility of different proportions of Phellodendron chinense and Atractylodes lancea.METHODS:HPLC method was used to determine contents of phellodendrine and berberine in different proportions of P.chinense and A.lancea.The determination was performed on Waters C18(250 mm×4.6 mm,5 μm) with mobile phase consisted of acetonitrile-0.1％ phosphoric acid (gradient elution).The detection wavelength was at 285 nm.RESULTS:The linear range of phellodendrine and berberine were 0.0206-0.4112 mg and 0.0211-0.4220 mg(both r=0.9997);RSDs of precision,stability and reproducibility tests were all lower than 1％.Average recoveries were 99.39％ (RSD=1.62％,n=6)and 99.51％ (RSD=1.05 ％,n =6).CONCLUSIONS:With A.lancea increased,the content of alkaloid decreased when P.chinense was compatible with A.lancea.关键词
药对/黄柏/苍术/配伍比例/生物碱含量Key words
Drug couple/Phellodendron chinense/Atractylodes lancea/Compatibility proportion/Alkaloid content分类
医药卫生引用本文复制引用
傅春升,王琰,李玥,张学顺..黄柏-苍术药对不同比例配伍中生物碱含量的变化[J].中国药房,2013,24(27):2549-2551,3.基金项目
山东省科技发展计划项目(No.2010GSF10284) （No.2010GSF10284）
