中国药房2013，Vol.24Issue(32)：3054-3055,2.DOI:10.6039/j.issn.1001-0408.2013.32.25
HPLC法测定知柏地黄丸中芒果苷的含量
Content Determination of Mangiferin in Zhibai Dihuang Pills by HPLC
邓树强 1陈彩瑜1
作者信息
- 1. 广东省惠东县人民医院,广东惠东 516300
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method for the content determination of mangiferin in Zhibai dihuang pills.METHODS:HPLC method was adopted.The determination was performed on Agilent ODS C18 column with mobile phase consisted of acetonitrile-0.2 ％ glacial acetic acid (15∶85,V/V) at the flow rate of 1.0 ml/min.The column temperature was 30 ℃,and the detection wavelength was set at 258 nm.The sample size was 10 μl.RESULTS:The linear range of mangiferin was 2.5-7.5 μg/ml(r=0.9998)with an average recovery of 99.45％ (RSD=0.75％,n=9).The RSDs of precision,slability and repeatability tests were all lower than 0.96％.CONCLUSIONS:The method is simple,rapid and accurate,which can be used for the content determination of mangiferin in Zhibai dihuang pills.关键词
知柏地黄丸/芒果苷/高效液相色谱法/含量测定Key words
Zhibai dihuang pills/Mangiferin/HPLC/Content determination分类
医药卫生引用本文复制引用
邓树强,陈彩瑜..HPLC法测定知柏地黄丸中芒果苷的含量[J].中国药房,2013,24(32):3054-3055,2.
