摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of dihydrotanshinone Ⅰ,cryptotanshinone,tanshinone Ⅰ and tanshinone ⅡA in Xianling gubao capsules.METHODS:HPLC method was adopted.The determination was performed on UltimateTM XB-C18 column with mobile phase consisted of 0.1％ formic acid-acetonitrile (gradient elution) at the flow rate of 1.0 ml/min.The detection wavelength was set at 270 nm,and sample size was 10 μl.The column temperature was 30 ℃.RESULTS:The linear ranges of dihydrotanshinone Ⅰ,cryptotanshinone,tanshinone Ⅰ,tanshinone Ⅱ A were 0.0532-5.32 μg/ml (r=0.9995),0.0512-5.12 μg/ml (r=0.9999),0.0508-5.08 μg/ml (r=0.9996),0.0537-5.37 μg/ml (r=0.9997),respectively.The average recoveries of four active ingredients were 97.93％ (RSD=0.72％,n=6),99.66％ (RSD=0.38％,n=6),99.02％ (RSD=0.54％,n=6) and 99.92％ (RSD=0.22％,n=6),respectively.RSDs of precision,stability and reproducibility tests were all lower than 1.42 ％.CONCLUSIONS:The method is rapid,accurate and repeatable,and can be used for quantitative determination of 4 liposoluble constituents in Xianling gubao capsules.关键词
仙灵骨葆胶囊/二氢丹参酮Ⅰ/隐丹参酮/丹参酮Ⅰ/丹参酮ⅡA/高效液相色谱法/含量测定Key words
KEY WORDS Xianling gubao capsules/Dihydrotanshinone Ⅰ/Cryptotanshinone/Tanshinone Ⅰ/Tanshinone Ⅱ A/HPLC/Content determination分类
医药卫生
