中国药房2013，Vol.24Issue(33)：3087-3089,3.DOI:10.6039/j.issn.1001-0408.2013.33.06
环孢菌素A血药浓度快速检测试纸的初步研制
Primary Preparation of Test Papers for Rapid Detection of Blood Concentration of Cyclosporin A
吕宗杰 1郑永 2程渝1
作者信息
- 1. 重庆市妇幼保健院,重庆400013
- 2. 重庆医科大学附属第二医院,重庆400010
- 折叠
摘要
Abstract
OBJECTIVE:To prepare test papers for rapid detection of blood concentration of cyclosporin A (CsA).METHODS:CsA monoclonal antibody was marked by colloidal gold and attached to fiberglas cellulose membranes,and then assembled into test papers; artificial antigen was solidified onto cellulose nitrate membranes,and then assembled into test papers.The detection range,limit of quantification,recovery,precision and stability of CsA were detected in plasma.The effects of different concentrations of azithromycin,lansoprazole,cefradine,ambroxol and glibenclamide on test papers were also determined.Results of 10plasma samples of CsA were compared between test paper and fluorescence polarization immunoassay.RESULTS:The detection range of CsA is 5-600 ng/ml by test papers (r=0.998 1).The limit of quantification was 5 ng/ml,and recovery rates were 86.51％-112.43％.RSDs of intra-day and inter-day were lower than 20％ (n=5).CsA was not stable within 15 d at room temperature,but it was stable within 3 months at 4 ℃.The blood concentrations of azithromycin,lansoprazole,cefradine,ambroxol and glibenclamide were all lower than 4 ng/ml and had no significant effect on test paper.There was no statistical significance in the determination results of 10 plasma samples by 2 methods (P＞0.05).CONCLUSIONS:Test papers for rapid detection of blood concentration of CsA have been developed successfully and show sound specificity.关键词
环孢菌素A/快速检测试纸/血药浓度/制备Key words
Cyclosporin A/ Rapid test papers/ Blood concentration/ Preparation分类
医药卫生引用本文复制引用
吕宗杰,郑永,程渝..环孢菌素A血药浓度快速检测试纸的初步研制[J].中国药房,2013,24(33):3087-3089,3.
