摘要
Abstract
OBJECTIVE:To investigate the effects of betaphrine on smooth muscle contraction of rat isolated uterus.METHODS:The isolated uterine smooth muscles samples of non-pregnant and pregnant rats were prepared.They were divided into non-pregnant blank (NPC) group,pregnant blank (PC) group,betaphrine non-pregnant (BNP) group,betaphrine pregnant (BP)group,ritodrine non-pregnant (RNP) group and ritodrine pregnant (RP) group (n=6).The concentration of medicine was accumulated every 15 min in those groups; by adding betaphrine,the concentration of medicine was accumulated to 10-10,10-9,10-8,10-7,10-6,and 10-5 mol/L in BNP group and BP group,respectively; by adding ritodrine,the concentration of medicine accumulated to 10-9,10-8,10-7,10-6,10-5 and 3×10-5 mol/L in RNP group and RP group,respectively; no medicine was given to NPC and PC group.The range of myometrial contraction was recorded within 105 min.Myometrial contraction rate,-lgIC50 (PIC50) and Emax were measured.RESULTS:Compared with NPC group and PC group,the contraction rate of smooth muscles deceased significantly in BNP group and BP group after 10-10-10-5 mol/L betaphrine was added in smooth muscles of corresponding model (P＜0.05),there was significant difference among different concentrations of betaphrine (P＜0.05) ; PIC50 of betaphrine in BNP group and BP group were 7.74 ± 0.12 and 7.92 ± 0.07,and Emax of betaphrine were (80.32 ± 5.49)％ and 100％,respectively.Compared with NPC group and PC group,the contraction rate of smooth muscles deceased significantly in RNP group and RP after 10-9-3× 10-5mol/L ritodrine was added in smooth muscles of corresponding model (P＜0.05),there was significant difference among different concentrations of ritodrine (P＜0.05) ; PIC50 of ritodrine in RNP group RP group were 7.28 ± 0.16 and 6.98 ± 0.13,and Emax of ritodrine were (63.63 ± 6.28) ％ and (65.48 ± 6.93) ％,respectively.CONCLUSIONS:Betaphrine and ritodrine can inhibit spontaneous contraction of isolated uterine smooth muscle in non-pregnant and pregnant rats,in concentration-dependent manner,and inhibitory effect of betaphrine is better than that of ritodrine.关键词
倍他福林/利托君/大鼠/未孕/妊娠/子宫收缩Key words
Betaphrine/ Ritodrine/ Rats/ Non-pregnant/ Pregnant/ Myometrial contraction分类
医药卫生
