摘要
Abstract
OBJECTIVE:To study inhibitory effects of Vitex negundo lignans-3 on the proliferation of human hepatic cancer HepG2 cells,and relationship of it with Akt and ERK1/2 signal pathway.METHODS:Human hepatic cancer HepG2 cells were as subject,after 48 h treatment the inhibitory rate of cell proliferation was determined by MTT assay in blank control group and 0.1,0.3,1.0,3.0,10.0 and 30.0 μmol/L Vitex negundo lignans-3 group (n=3); the viable count of cells were determined by cell count assay in Vitex negundo lignans-3 (10.0 μmol/L) group,5-FU (10.0 μmol/L) group,solvent (complete medium containing 0.1％DMSO) group and blank control group (n=3) after treated for 24,48 and 72 h; after 24 h treatment,the expressions of p-Akt and p-ERK1/2 protein were determined by Western blotting assay in solvent group,Vitex negundo lignans-3 (10.0 μmol/L) group,phosphatidylinositol-3-kinase blocker LY294002 (10.0 μmol/L) group and mixture group (n=3).RESULTS:Compared with blank control group,the inhibitory rate of cells increased significantly in 1.0-30.0 μmol/L Vitex negundo lignans-3 groups (P＜0.01) ;compared with solvent group and blank control group,the viable count of cells decreased significantly in Vitex negundo lignans-3group after treated for different time (P＜0.01); compared with 5-FU group,the viable count of cells increased significantly in Vitex negundo lignans-3 group after treated for 72 h (P＜0.01); compared with solvent group,the expressions of p-Akt and p-ERK1/2 protien decreased significantly in Vitex negundo lignans-3 group,LY294002 group and mixture group (P＜0.01),but there was no significant difference among the latter 3 groups.CONCLUSIONS:Vitex negundo lignans-3 inhibits the proliferation of HepG2 cells in concentration and time-dependent manner,associating with the inhibition of Akt and ERK1/2 protein phosphorylation.关键词
人肝癌HepG2细胞/黄荆子木脂素3/细胞增殖/丝氨酸-苏氨酸蛋白激酶/细胞外调节蛋白激酶1/2/蛋白表达Key words
Human hepatic cancer HepG2 cells/ Vitex negundo lignans-3/ Cell proliferation/ Akt/ ERK1/2/ Protein expression分类
医药卫生
