中国药房2013，Vol.24Issue(33)：3102-3104,3.DOI:10.6039/j.issn.1001-0408.2013.33.11
二甲双胍对去卵巢模型大鼠血清中瘦素含量的影响及作用机制
Effects of Metformin on the Serum Content of Leptin in Ovariectormized Model Rats and Its Mechanism
王志新 1康白1
作者信息
- 1. 潍坊医学院应用药理学实验室,山东潍坊261053
- 折叠
摘要
Abstract
OBJECTIVE:To study the effects of metformin (MF) on the serum content of leptin in ovariectormized model rats and its mechanism.METHODS:Female rats underwent ovariosteresis surgery to establish ovariectomized model.Model rats were randomly divided into model group,MF low-dose and high-dose groups [135,270 mg/(kg,d)] with 10 rats in each group; other 10 healthy female rats underwent sham operation were sham group.Model group and sham group were given 0.9％ Sodium chloride 5 ml/(kg·d) intragastrically,and MF low-dose and high-dose groups were given relevant drugs intragastrically for consecutive 42 days.The serum content of leptin was measured with radioimmunoassay and body weight of rats were measured; the expression of SOCS-3 mRNA in lipid was measured with RT-PCR.RESULTS:Compared with sham group,the serum levels of leptin,body weight and the expression of adipose SOCS-3 mRNA increased significantly in model group (P＜0.05 or P＜0.01).Compared with model group,the serum levels of leptin,body weight and the expression of adipose SOCS-3 mRNA decreased significantly in MF high-dose group (P＜0.05) ; but above index had no statistical significance in MF low-dose group (P＞0.05).CONCLUSIONS:The high-dose of MF can significantly decrease the serum levels of leptin in ovariectormized model rats,and it may be related to the down-regulation of the expression of SOCS-3 mRNA.关键词
二甲双胍/去卵巢/大鼠/瘦素/细胞因子信号传导抑制因子3Key words
Metformin/ Ovariectormized/ Rats/ Leptin/ Suppressor of cytokine signaling 3分类
医药卫生引用本文复制引用
王志新,康白..二甲双胍对去卵巢模型大鼠血清中瘦素含量的影响及作用机制[J].中国药房,2013,24(33):3102-3104,3.
