摘要
Abstract
OBJECTIVE:To develop a method for the determination of leucogen in rat plasma,and to study its pharmacokinetics.METHODS:Rats were given leucogen 1 mg/kg by tail intravenous administration and leucogen 10 mg/kg by intragastric administration (n=6).Using tinidazole as internal standard,LC-MS method was used to detect the plasma concentration of leucogen before and 10,20,30,40,60,90,120,180,240,360,1 440 min after medication; pharmacokinetic parameters were calculated with DAS 2.0 software.The determination was performed on a Agilent Zorbax Eclipse plus C18 column with mobile phase consisted of acetonitrile-water-acetic acid (20 ∶ 80 ∶ 0.3) for gradient elution.MS condition:ESI,296.1 [M+H]+ for leucogen,248.1[M+H]+for tinidazole.RESULTS:The linear range of leucogen were 0.048-30 μg/ml (r=0.999 3).The limit of quantification of leucogen was 0.048 μg/ml.Pharmacokinetic parameters of leucogen after tail intravenous administration vs.intragastric administration were as follows as cmax:(1.51 ± 0.66) vs.(17.78 ± 2.67) μg/ml; t1/2z:(1.23 ± 0.85) vs.(0.88 ± 0.23) h; AUC0-∞:(1.29 ± 0.56) vs.(9.87 ±0.47) μg· h/ml.CONCLUSIONS:The method is accurate,reliable,sensitive for detection and suitable for plasma concentration monitoring of leucogen.Pharmacokinetics of leucogen is in line with one-compartment model.关键词
液质联用法/利可君/血药浓度/大鼠/药动学Key words
LC-MS/ Leucogen/ Plasma concentration/ Rats/ Pharmacokinetics分类
医药卫生
