中药材2013，Vol.36Issue(6)：958-961,4.
龙葵对人结肠癌RKO细胞粘附、移动和侵袭的影响
Effect of Solanum nigrum on Human Colon Carcinoma RKO Cells
摘要
Abstract
Objective:To evaluate the effect of Solanum nigrum on adhesion,migration and invasion in human colon carcinoma RKO cells.Methods:RKO cells were treated with different dose of Solanum nigrum.Cell proliferation was detected by CCK-8 assay.Cell adhesion was observed with CytoSelectTM 48-Well Cell Adhesion Assay.Cell migration was detected with scratch assay.Cell invasion was analyzed by CytoSelectTM 24-Well Cell Invasion Assay.Results:At final concentration of 400 ～ 1 600 μg/mL,Solanum nigrum significantly inhibited proliferation of RKO cells in a dose-dependent manner.At final concentration of 100 ～400 μg/mL,Solanum nigrum significantly inhibited adhesion,migration and invasion in RKO cells.Conclusion:Solanum nigrum may inhibit the proliferation,adhesion,migration and invasive abilities in RKO cells.The present study provides new insight into the application of Solanum nigrum for colon carcinoma treatment that are worthy of further study.关键词
结肠癌/龙葵/增殖/粘附/移动/侵袭Key words
Colon carcinoma cell / Solanum nigrum Linn./ Proliferation/ Adhesion/ Migration / Invasion分类
医药卫生引用本文复制引用
胡兵,安红梅,沈克平,史秀峰,邓珊,魏蒙蒙..龙葵对人结肠癌RKO细胞粘附、移动和侵袭的影响[J].中药材,2013,36(6):958-961,4.基金项目
国家自然科学基金(81273726) （81273726）
上海市中医药事业三年行动计划首批重大研究项目(ZYSNXD-CC-ZDYJ017) （ZYSNXD-CC-ZDYJ017）
国家中医管理局中医肿瘤重点学科合作项目(LHZLK-1107) （LHZLK-1107）
