中药材2013，Vol.36Issue(6)：994-998,5.
知母皂苷BⅡ的提取及大孔树脂纯化工艺研究
Study on the Extraction Process and Macroporous Resin for Purification of Timosaponin B Ⅱ
摘要
Abstract
Objective:To optimize the extraction process and macroporous resin for purification of Timosaponin B Ⅱ from Anemarrhena asphodeloides.Methods:Orthogonal design L9 (34) was employed to optimize the circumfluence extraction conditions by taking the extraction yield of Timosaponin B Ⅱ as index.The absorption-desorption characteristics of eight kinds of macroporous resins were evaluated,then the best resin was chosen to optimize the purification process conditions.Results:The optimum extraction conditions were as follows:the herb was extracted for 2 times (2 hours each time) with 8.5-fold 50％ ethanol at the first time and 6-fold 50％ ethanol at the second time.HPD100 resin showed a good property for the absorption-desorption of Timosaponin B Ⅱ.The optimum technological conditions of HPD100 resin were as follows:the solution concentration was 0.23 mg/mL,the amount of saturated adsorption at 4/5 body volumn (BV) resin,the HPD100 resin was washed with 3 BV water and 6 BV 20％ ethanol solution to remove the impurity,then the Timosaponin B Ⅱ was desorbed by 5 BV ethanol solution.The purity of Timosaponin B Ⅱ was about 50％.Conclusion:The optimized extraction process and purification is stable,efficient and suitable for industrial production.关键词
知母/知母皂苷BⅡ/提取工艺/正交试验/HPD-100大孔树脂吸附Key words
Anemarrhena asphodeloides Bge./Timosaponin BⅡ/ Extraction process/Orthogonal design/HPD 100 macroporous resin分类
医药卫生引用本文复制引用
刘艳平,丁越,张彤,王冰,蔡贞贞,陶建生..知母皂苷BⅡ的提取及大孔树脂纯化工艺研究[J].中药材,2013,36(6):994-998,5.基金项目
国家自然科学基金(81173561) （81173561）
教育部新世纪人才计划(NCET-10-0944) （NCET-10-0944）
上海市教委曙光计划及创新团队(10SG40) （10SG40）
