基础医学与临床2011，Vol.31Issue(2)：144-148,5.
乙型肝炎病毒X蛋白通过激活ERKs和NF-κB上调大鼠系膜细胞表达TNF-α
Hepatitis B virus X protein upregulates tumor necrosis factor-α expression of rat mesangial cell line via ERKs and NF-KB pathway
摘要
Abstract
Objective To investigate the signal transduction pathway of hepatitis B virus X protein (HBx) in glomerular mesangial cell(GMC) TNF-α expression.Methods pCI-neo-X was transfected into glomerular mesangial cells.TNF-α and its mRNA expression of GMC were compared in animals treated or untreated with ERKs inhibitor U0126, NF-κB inhibitor lactacystin and P38 inhibitor SB203580, respectively.HBx expression in glomerular mesangial cells was assessed by Western blot.TNF-α mRNA expression was assessed by semi-quantitative RT-PCR.TNF-α levels in supematants were measured by ELISA.Results HBx expression was found in transfected glomerular mesangial cells, and became prominent at 36 and 48 hours after transfection whatever with or without inhibitors in culture media.TNF - α mRNA expression significantly decreased in U0126 or lactacytin group compared with U0126-free or lactacytin-free group.TNF-α levels in supematants in pCI-neo-X transfection was also partially inhibited by ERKs inhibitor U0126 or NF-κB inhibitor lactacystin, but no effect was observed in P38 inhibitor SB203580 treated group at 36 and 48 h after transfection.Conclusion HBx upregulated TNF-α expression of GMC line partly through ERK1/2 and NF-κB signal transduction pathway, but not P38 MAPK pathway.关键词
乙型肝炎病毒/X蛋白/系膜细胞/细胞外蛋白调节激酶/肿瘤坏死因子α/核因子-κBKey words
heptitis B virus/ X protein/ mesangial cell line/ ERKs/ tumor necrosis factor-α/ NF-κB分类
医药卫生引用本文复制引用
卢宏柱,樊启红,刘丹,邓开琴,周建华..乙型肝炎病毒X蛋白通过激活ERKs和NF-κB上调大鼠系膜细胞表达TNF-α[J].基础医学与临床,2011,31(2):144-148,5.基金项目
湖北省卫生厅计划课题(JX4B47) （JX4B47）
长江大学博士启动基金(2009001) （2009001）
