基础医学与临床2011，Vol.31Issue(2)：179-182,4.
褪黑素促进哮喘小鼠肺组织STAT4的表达及抑制气道炎症
Melatonin promotes the expression of STAT4 and inhibite airway inflammation in asthmatic mouse
摘要
Abstract
Objective To investigate the effect of melatonin (MT) on signal transduction and activation on tran scription 4 (STAT4) and impact on airway inflammation of asthmatic mice. Methods One hour after the last aerosol exposure, the left lungs were lavaged, the number of inflammatory cells in the mouse bronchoaleolar lavage fluids (BALF) were counted with microscope. Using immunohistochemistry and real-time fluorescent quantitative PCR to detect the expression of STAT4 protein and its mRNA expressionism in right lung tissue. Using enzyme-linked im munosorbent assay to detecte levels of IL-12 in serum. Results ( 1 ) In test group, the number of inflammatory cells and EOS were significantly higher than control group, STAT4 protein and STAT4 mRNA expression in lung tis sue was significantly decreased when compared with control group. MT treated group significantly alleviated the above-mentioned parameters. And there were significant differences between MT treated group and control group, DXM treated group and model group. (P ＜0.01 ). (2)In asthmatic mice, STAT4 protein and its mRNA expres sion had both altitude negative relationship with EOS counts in BALF (r =0.754, P ＜0. 01; r =0. 755, P ＜0. 01, respectively). Levels of IL-12 in serum had altitude positive correlation with STAT4 protein expression in asthmatic group mice( r = 0.742,P ＜ 0.01 ). Conclusion MT may induce STAT4 gene transcription and translation in asth matic mice, so promotes production of IL-12 in peripheral blood and inhibits inflammatory cell infiltration.关键词
STAT4/褪黑素/哮喘/气道炎症分类
医药卫生引用本文复制引用
王敏,李美容,张光环,熊安秀..褪黑素促进哮喘小鼠肺组织STAT4的表达及抑制气道炎症[J].基础医学与临床,2011,31(2):179-182,4.基金项目
宜昌市2010年医疗卫生科技计划(A01301-09) （A01301-09）
