基础医学与临床2011，Vol.31Issue(3)：247-251,5.
NF-κB参与七氟烷预处理减轻大鼠心肌缺血再灌注损伤
NF-kB participates the process of sevoflurane preconditioning on myocardium against ischemia-reperfusion injury in rats
摘要
Abstract
Objective To investigate the protective effects of NF-KB P65 active expression during sevoflurane preconditioning on rat myocardium against ischemia-reperfusion injury in vivo. Methods Seventy-eight male adult SD rats weighing 270 ～ 390 g were anesthetized with intraperitoneal pentobarbital sodium 50 mg/kg, tracheostomized and mechanically ventilated. PaCO2 was maintained at 25 ～40 mmHg( 1 mmHg =0. 133 kPa). I/R was produced by reversible occlusion of left anterior descending branch (LAD) of coronary artery for 30 min followed by 2 h reperfusion. Myocardium samples of all groups were collected before the time of myocardial ischemia and after the time of myocardial ischemia reperfusion respectively. In addition, (G) Sevoflurane group; Rats were received 2. 5 ％ sevoflurane for 30 min and then collected myocardium sample after 165 min. NF-KB P65 was determined by Western blot analysis. Results The expression of NF-KB P65 protein was significantly up-regulated in sevoflurane-I/R group as compared to that of the sham group. The expression of NF-kB P65 protein was significantly up-regulated in simple-ischemic group than that in the sham group and the expression of NF-KB P65 protein was significantly downregulated in sevoflurane-I/R group, sham group, SEVO group, PTN group, SEVO-I/R + PTN group and PTN + SEVO-I/R group as compared to that of simple-ischemic group. Conclusion Sevoflurane preconditioning has protective effects on ischemia-reperfusion myocardial injury in rats. This protective effect may be involved in the activation of NF-KB P65 during sevoflurane preconditioning.关键词
核转录因子-κB/七氟烷/预处理/心肌缺血再灌注损伤Key words
nuclear factor-kappa B/ sevoflurane/ preconditioning/ myocardium ischemia-reperfusion injury分类
医药卫生引用本文复制引用
覃琴,谢红,王琛,刘霞,朱江,吴雪梅..NF-κB参与七氟烷预处理减轻大鼠心肌缺血再灌注损伤[J].基础医学与临床,2011,31(3):247-251,5.基金项目
国家自然科学基金(30872453) （30872453）
江苏省自然科学基金(BK2008166) （BK2008166）
江苏省高校自然科学基础研究面上项目(08KJD32005) （08KJD32005）
苏州市第十三批科技发展计划(社会发展及医药)(2008-11) （社会发展及医药）
