基础医学与临床2011，Vol.31Issue(3)：268-274,7.
人脐带间充质干细胞诱导分化为神经元样细胞
Neural induction of human umbilical cord mesenchymal stem cells
摘要
Abstract
Objective To investigate the potential of human umbilical cord mesenchymal stem cells (hUC-MSCs)differentiating into neural cells through in vitro induction. Methods Using three methods, including growth factor bFGF and EGF associated with retinoic acid, traditional Chinese medicine monomer ursolic acid( UA), the supernatant of the neurons from cerebral cortex of newborn rat cultured in serum-free to induce hUC-MSCs to differentiate into neural cells, observe the morphological changes and detect the special markers of neural cells by immunocytochemistry. Results Induced by cytokines, UA(2 mg/L), hUC-MSCs gradually become bipolar, multipolar or conical instead of fibrous, and the body also shrinked. Some cells showed sphericity with short processes. Induced by the supernatant of the neurons from cerebral cortex of newborn rat cultured in serum-free media, hUCMSCs may firstly differentiate into Nestin and Musashi-1 positive neurospheres, then undergo the morphological changes mentioned above and form neural cells. Shown by immunocytochemistry, a small quantity of hUC-MSCs express Tubulin, GFAP, or 04 after induction of 14 days. Conclusion hUC-MSCs can be induced in vitro to differentiate into neural cells under certain conditions, providing a new approach for cell transplantation treatment of nervous system diseases.关键词
脐带间充质干细胞/诱导分化/神经细胞Key words
hUC-MSCs/ differentiation/ neural cells分类
医药卫生引用本文复制引用
程欣,皮婷,肖飞,马刘红,徐建兵,罗焕敏..人脐带间充质干细胞诱导分化为神经元样细胞[J].基础医学与临床,2011,31(3):268-274,7.基金项目
广州市科技计划(10C32070469) （10C32070469）
中央高校基本科研业务费专项资金(21609502) （21609502）
暨南大学2009年国家大学生创新性实验计划(091055929) （091055929）
