基础医学与临床2011，Vol.31Issue(3)：275-280,6.
Annexin A3抑制顺铂诱导人卵巢上皮癌细胞凋亡
Annexin A3 inhibits apoptosis induced by cisplatin in human epithelial ovarian cancer cells
摘要
Abstract
Objective To determine whether annexin A3 could regulate cisplatin sensitivity through apoptosis induced by cisplatin or not. Methods Retroviral vector constuction and cell infection technology were used to obtain high-level annexin A3 and low-level annexin A3 ovarian cancer cells. Then Annexin V/propidium iodide analysis and Western blot were used to determine the cell apoptosis rate and caspase cleavage. Results When annexin A3 level was up-regualted, the average apoptosis rate induced by 60 μg/mL cisplatin in sensitive cells decreased from 68. 72％ ± 1.01％ to 29. 13％ ±2. 61％ (P ＜0. 05), cleavages of caspase and PARP were induced by high level cisplatin. Conversely, in cisplatin resistant cells with low-level annexin A3, the average apoptosis rate was upreguated from 35. 05％ ± 3.06％ to 76. 73％ ± 6. 42％ (P ＜ 0. 05 ), low level cisplatin stimulated cleavages of caspase and PARP. Besides, annexin A3 may delay phosphorylation of P38 MAPK in ovarian cancer cells. Conclusion All these results show that apoptosis inhibition is a potential mechanism of annexin A3 regulating platinum resistance in ovarian cancer cells.关键词
卵巢癌/annexin A3/凋亡/顺铂/耐药Key words
ovarian cancer/ annexin A3/ apoptosis/ cisplatin/ drug resistance分类
医药卫生引用本文复制引用
尹婕,张永莉,姚欣,王晓艳,毛宁,潘凌亚..Annexin A3抑制顺铂诱导人卵巢上皮癌细胞凋亡[J].基础医学与临床,2011,31(3):275-280,6.基金项目
北京协和医院重点基金(200203) （200203）
