扬州大学学报（农业与生命科学版）2013，Vol.34Issue(3)：33-38,6.
FSHβ基因5'调控区多态性与小尾寒羊产羔数的关系
Polymorphism of 5'regulatory region of FSHβ gene and its relationship with litter size of Small Tail Han sheep
摘要
Abstract
The FSH gene was studied as a candidate gene for prolificacy in Small Tail Han sheep.Single nucleotide polymorphisms (SNPs) of about 1.5 kb sequence of the proximal 5'regulatory region of follicle-stimulating hormone FSH gene β subunit were detected by polymerase chain reaction-single strand conformation polymorphism.The result indicated that the product amplified by primer P3,P5 and P6 displayed polymorphism.Three genotypes were detected in each primer.Sequencing revealed that there were two alleles (C and D) of product amplified by primer P3.Compared with wild type,there was one three-base mutation (TCG→GTC) at-899 bp of FSHβ gene in the D allele.Besides this mutation,a single nucleotide mutation (T→A) at-933 bp in the C allele was also detected.Two couples of alleles (A and B,E and F) showed in product amplified by primer P5 and primer P6.Sequences of B allele and F allele were completely consistent with GenBank,which were defined as wild type; while there were three single-base mutations (A-438G,T-411C and T-375G) in the A allele,and one single-base mutations (T-181C) in the E allele.In addition,a single base deletion (T) at-402 bp was also found in the A allele.Statistic results revealed that A,C,E allele frequencies were significantly higher than the B,D,F allele frequency.AA,CC,EE genotype frequencies were higher than AB,CD,EF genotype frequencies,and BB,DD,FF genotype frequencies were the lowest ones in Small Tail Han sheep.Statistics results showed that it varied most in primer P3 and varied least in primer P5.The least squares value of litter size in Small Tail Han sheep were AA＞AB＞BB,CC＞CD＞DD,EE＞EF＞FF,but differences of LSM value of litter size between those genotypes were not significant (P＞0.05).关键词
小尾寒羊/促卵泡素β亚基基因/PCR-SSCP/产羔数Key words
Small Tail Han sheep/ follicle-stimulating hormoneβ gene/ PCR-SSCP/ litter size分类
农业科技引用本文复制引用
吴舒洁,储明星,过玮,王凭青,吴常信,狄冉,刘秋月,李宁..FSHβ基因5'调控区多态性与小尾寒羊产羔数的关系[J].扬州大学学报（农业与生命科学版）,2013,34(3):33-38,6.基金项目
国家肉羊产业技术体系专项(CARS-39) （CARS-39）
