扬州大学学报(农业与生命科学版)2013,Vol.34Issue(3):44-48,5.
油菜外源抗除草剂基因的分子检测
Molecule detection of herbicide-resistant transgenic rapeseed
摘要
Abstract
In this paper,three unknown rapeseed varieties were detected through conventional PCR and real-time fluorescent PCR with primers and probes designed according to the sequences of BAR gene and GOX gene.The results showed that variety A was non-transgenic,variety B was glufosinate-resistant and variety C was glyphosate-resistant.This work provides technical support for inspection and testing of GMO biosafety.关键词
油菜/抗除草剂/转基因检测/PCRKey words
rapeseed/ herbicide resistant/ transgenic detection/ PCR分类
农业科技引用本文复制引用
赵祥祥,陈桂敏,刘福霞,唐瑭,卜翠萍,赵祥强..油菜外源抗除草剂基因的分子检测[J].扬州大学学报(农业与生命科学版),2013,34(3):44-48,5.基金项目
国家自然科学基金资助项目(30900217) (30900217)
江苏省自然科学基金资助项目(BK2011410) (BK2011410)
江苏省"青蓝工程"中青年学术带头人培养对象资助项目 ()