中国农业科学2013，Vol.46Issue(17)：3534-3544,11.DOI:10.3864/j.issn.0578-1752.2013.17.002
小豆SSR分子标记遗传连锁图谱构建
Construction of Genetic Linkage Map Using SSR Molecular Markers in Azuki Bean (Vigna angularis Ohwi and Ohashi)
摘要
Abstract
[Objective] The published SSR primers of cowpea,common bean and EST-SSR (expressed sequence tags) primers of common bean were located and integrated in azuki bean linkage group to construct a linkage map of Chinese azuki bean with azuki bean SSR primers as anchor markers.More practical molecular markers will be supplied to gene mapping,cloning and molecular marker-assisted selection ofazuki bean.[Method] A total of 1 473 SSR and EST-SSR primer pairs,including 906 SSR primer pairs of cowpea,123 SSR and 248 EST-SSR of common bean,196 SSR primer pairs of azuki bean,were used for PCR amplification to screen polymorphic markers between cultivated azuki bean parents of HB801 ×AG109 and GM892×AG110.Their F2 segregating populations were tested with the polymorphic markers.[Result] An integrated genetic linkage map ofazuki bean containing 145 SSR and EST-SSR markers was constructed.This molecular genetic linkage map is composed of 59 azuki bean SSR anchoring markers,newly mapped 63 cowpea SSR markers,9 common bean SSR markers and 14 common bean EST-SSR markers,as well as a stem colour marker-the purple stem trait which is located on linkage group 9.Genetic distance of purple stem trait from CEDG022 and cbess058 molecular markers is 0.9 cM and 0.1 cM,respectively.The total length of the linkage map was 823 cM and covered 11 linkage groups.The average distance between markers was 5.64 cM.The average distance of each linkage group spanned 74.82 cM.The average number of markers was 13.27 for each of 11 chromosomes.The length of each linkage group ranged from 7 to 26 markers was from 49.1 cM to 125.6 cM.[Conclusion] Molecular markers of the relative species were freshly introduced in azuki bean and increased density of its genetic linkage map.It is useful for gene mapping and cloning,molecular marker-assisted selection in azuki bean.关键词
小豆/SSR/EST-SSR/遗传连锁图谱Key words
azuki bean (Vigna angularis Ohwi & Ohashi)/ SSR/ EST-SSR/ genetic linkage map引用本文复制引用
骆晚侠,张李,杨凯,李奕松,赵波,李明,万平..小豆SSR分子标记遗传连锁图谱构建[J].中国农业科学,2013,46(17):3534-3544,11.基金项目
国家自然科学基金项目(31071474)、北京市教委2011年高级别大项目匹配(1086716024)、2012科研质量提高-生物种业支持项目(1086716066) （31071474）
