中国农业科学2013，Vol.46Issue(17)：3669-3679,11.DOI:10.3864/j.issn.0578-1752.2013.17.016
燕麦发芽前后酚类物质的组成变化及结构鉴定
Composition Change and Structure Identification of Phenols in Oat After Germination
摘要
Abstract
[Objective] Changes of phenols composition after oat germination and structure identification of possible avenanthramides were studied.[Method] HPLC was used to analyze the phenol composition of extracts from ungerminated oat seeds,6-day germinated oat grains,buds and roots.HPLC/Q-TOF-MS was chosen to detect the molecular weight of avenanthramides.[Result] The results indicated that the kind and content of phenolic acid in 6-day germinated oat grains were reduced.The relative content of phenolic acid was reduced from 32.14％ to lower than 5％,while the relative content avenanthramide was increased from 45.52％ to 79.21％.A group of avenanthramides such as 2c,2p,2f,t2,and t3 were both in ungerminated oat seeds and 6-day germinated oat grains.The molecular formula (molecular weight) of 2c,2p,2f,t2 and t3 were,respectively,C16H13O6N(315.0753),C16H13O5N(299.0818),C17H15O6N(329.0912),C18H15O5N(325.0968) and C19H17O6N(355.1077).Avenanthramides t2 and t3 increased drastically during oat germination.The composition of 6-day germinated oat buds and roots extracts was different from oat grain extract.The molecular weight of w6-w10 were,respectively,564.1498,592.1808,494.1781,710.1834 and 192.0433.[Conclusion] Phenol composition changed obviously after oat germination.The structure of six avenanthramides and the molecular weight of four possible avenanthramides were identified,thus offering a theoretical theory evidence for the study of germinated oat.关键词
燕麦/发芽/酚类物质/结构Key words
oat/ germination/ phenols/ structure引用本文复制引用
付晓燕,吴茜,李书艺,肖娟,谢笔钧,孙智达..燕麦发芽前后酚类物质的组成变化及结构鉴定[J].中国农业科学,2013,46(17):3669-3679,11.基金项目
"十一五"国家科技支撑计划项目(2006BAD27B09) （2006BAD27B09）
