中国农业科学2013，Vol.46Issue(17)：3721-3728,8.DOI:10.3864/j.issn.0578-1752.2013.17.022
婚飞对中华蜜蜂性成熟处女蜂王sRNAs表达的影响
Effects of Mating Flight on sRNAs Expression in Sexual Matured Virgin Queens (Apis cerana cerana)
摘要
Abstract
[Objective] The objective of this study is to analyze the influence of flight behaviour on sRNAs expression in matured virgin queens (Apis cerana cerana).[Method] The sexual matured virgin queens of A.cerana cerana were used as flying group that allow to fly in a certain area and the other group was not.The sRNAs expression differences between the two groups were analyzed by using a high-throughput sequencing method.[Result] Both of the flying matured virgin queens and non-flying counterparts had a complicated sRNAs population,and that the length of sRNAs varied,22 nt and 27-29 nt being the predominant length,while the frequence percent of the same RNA was different in the two samples.Combining deep sequencing and bioinformatics analysis,the percent of the common sequences in total sRNAs was 92.79％,but the number of unique sRNAs in the flying queens was more than that in non-flying queens.Matching to known miRNA alignment,a total of 25 known miRNAs were significantly different between them,with 1 up-regulated and 24 down-regulated in the flying matured virgin queens while there were 1 1 target genes of 19 known miRNA which were expressed differentially between them.[Conclusion]There are a large number of sRNAs changes during the flight process of matured virgin queens which may play an important role in adjusting physiology for mating successfully of virgin queens.关键词
中华蜜蜂/蜂王/飞行/miRNAKey words
Apis cerana cerana / queen bee/ flight / miRNA引用本文复制引用
吴小波,王子龙,石元元,张飞,曾志将..婚飞对中华蜜蜂性成熟处女蜂王sRNAs表达的影响[J].中国农业科学,2013,46(17):3721-3728,8.基金项目
国家自然科学基金项目(31060327)、江西农业大学青年科学基金项目(2010-09003303)、江西省研究生创新基金项目(YC2011-B016) （31060327）
