江苏大学学报（医学版）2014，Vol.24Issue(1)：1-5,5.DOI:10.13312/j.issn.1671-7783.y130253
血管内皮祖细胞移植对大鼠重症急性胰腺炎肾损伤的保护作用
Protecting effect of endothelial progenitor cell transplantation on renal injury with severe acute pancreatitis
摘要
Abstract
Objective:To explore the protecting effect of endothelial progenitor cells(EPCs) transplantation on renal complicated injury with severe acute pancreatitis(SAP).Methods:To manufacture the superparamagnetic iron oxide(SPIO) by the coprecipitation method; collecting rat EPCs from bone marrow,mononuclear cells were isolated with ficoll,VEGF,fibroblast growth factor (FGF) in the culture medium ; EPCs were labeled by the SPIO; 72 rats were randomly divided into control group,SAP group,SPIO-EPCs group.The SAP models were established by injection of sodium taurocholate into the pancreatic capsule.Rats in the SPIO-EPCs group were injected with SPIO-EPCs through tail vein.Rats in SAP group and control group only received phosphate buffered solution with the same dose.Then the serum levels of AMS,BUN,Cr and TNF-α at 2 h,6 h,12 h after modeling.Pathologic alteration of kidney were also observed.HE staining was used to observe the pathologic change of kidney tissue on different time between three groups.Prussian blue staining was used to observe the change of iron particles in SPIO-EPCs group on different time.Results:In SAP group serum levels of AMS,BUN,Cr and TNF-α were significantly elevated compared with control group (P ＜0.01).Compared with SAP group,serum levels of AMS,BUN,Cr and TNF-α were significantly decreased in SPIO-EPCs group (P ＜ 0.01).The pathological scores of renal in SAP group were higher than those of control group (P ＜ 0.05).The pathological scores of renal in SPIO-EPCs group were lower than those of SAP group(P ＜0.05).From the Prussian blue staining,we can observe that the iron particles in renal vascular endothelial increased gradually on the time passed.Conclusion:EPCs transplantation can reduce the release of inflammatory mediators,reduce renal vascular endothelial injury SAP.The transplanting EPCs can alleviate the renal injury with SAP.关键词
内皮祖细胞/重症急性胰腺炎/肾损伤/超顺磁性氧化铁/大鼠Key words
endothelial progenitor cells/ severe acute pancreatitis/ renal injury/ superparamagnetic iron oxide / rat分类
医药卫生引用本文复制引用
党胜春,王平江,曾艳华,陈荣芳,王豪,冯舒,崔磊,张建新..血管内皮祖细胞移植对大鼠重症急性胰腺炎肾损伤的保护作用[J].江苏大学学报（医学版）,2014,24(1):1-5,5.基金项目
国家自然科学基金资助项目(81070287) （81070287）
江苏省自然科学基金资助项目(BK2011484,BK2012704) （BK2011484,BK2012704）
