江苏大学学报（医学版）2014，Vol.24Issue(1)：48-51,4.DOI:10.13312/j.issn.1671-7783.y130194
慢性乙型肝炎患者外周血调节性B细胞与调节性T细胞的表达频率及其相关性
Expression and roles of regulatory B cells and T cells in patients with chronic hepatitis B
摘要
Abstract
Objective:To investigate the expression and roles of IL-10+ CD19+ regulatory B cells (Breg) and CD4 + CD25high regulatory T cell(Treg) in patients with chronic hepatitis B.Methods:A total of 28 patients with acute hepatitis B (AHB),31 patients with chronic hepatitis B (CHB) and 25 normal subjects(HCs group) were enrolled in this study.Peripheral blood mononuclear cells(PBMC) were isolated and stimulated with CpG ODN 2006 and PMA.The expression of IL-10 + CD19 + Breg and CD4 + CD25high Treg was analyzed with flow cytometry.The level of IL-10 in culture supematant was analyzed with ELISA.CD4 + CD25 + T cells were removed with Immunomagnetic beads to analyze the interactions between Breg and Treg.Results:After stimulation with CpG ODN 2006 and PMA,compared with the AHB group and the HCs group,percentages of Breg and Treg in the CHB group increased significantly (Breg:P =0.003,0.001 ;Treg:P ＜0.001,＜0.005),and no difference was found between the AHB group and the HCs group (Breg:P=0.796; Treg:P =0.227).Spearman correlation analysis found that in the CHB group,Breg and Treg was positively correlated (r =0.50,P =0.004),but in the AHB group and the HCs group,there was no such correlation (r =-0.15,P =0.462;r =0.09,P =0.669).IL-10 expression in the CHB group was higher than that in the AHB group and the HCs group (Z =-6.596,-6.373,P ＜ 0.001),and the difference between the AHB group and the HCs group was not statistically significant (Z =-0.952,P =0.341).Spearman correlation analysis showed that in the CHB group,IL-10 and Breg were positively correlated (r =3.303,P =0.005).Before and after removal of CD4 + CD25 + T cells,in the CHB group,the difference between Breg and IL-10 expression was not statistically significant.Conelusion:The expression of Breg and Treg increased in patients with chronic hepatitis B,Breg might play a regulatory role by IL-10 secretion.关键词
调节性B细胞/慢性乙型肝炎/调节性T细胞/IL-10Key words
regulatory B cells/ chronic hepatitis B/ regulatory T cells/ IL-10分类
医药卫生引用本文复制引用
杨鸿林,金洁,蒋廷旺..慢性乙型肝炎患者外周血调节性B细胞与调节性T细胞的表达频率及其相关性[J].江苏大学学报（医学版）,2014,24(1):48-51,4.基金项目
常熟市科技发展计划(社会发展)项目(CS201113) （社会发展）
