江苏大学学报（医学版）2014，Vol.24Issue(1)：63-67,71,6.DOI:10.13312/j.issn.1671-7783.y130036
急性冠脉综合征外周血单核细胞Toll样受体4、锌指蛋白A20与组织因子的表达及意义
Research on the correlation of TLR4, zinc finger protein A20 in peripheral blood monocyte and tissue factor in acute coronary syndrome patients
于春杨 1王昭军 1蒲肖君 1陈小节1
作者信息
- 1. 江苏大学附属医院心内科,江苏镇江212001
- 折叠
摘要
Abstract
Objective:To investigate the relation between the expression levels of toll-like 4/A20 mRNA in peripheral blood mononuclear cells(PBMC) and tissue factor,tissue factor passway inhibitor (TFPI) in plasma of patients with acute coronary syndrome (ACS).Methods:The patients of coronary artery diseases (CAD) were divided into the group of ACS (n =31),there were 15 patients with acute myocardial infarction (AMI),and 16 patients with unstable angina pectoris(UAP); and stable angina pectoris(SAP,n =21) group; the group of control were a total of 20 cases.The expressions of TLR4 and A20 mRNA were detected by real-time fluorescence quantitation polymerase chain reaction (RT-PCR).The concentrations of plasma tissue factor and TFPI were measured by enzyme-linked immunosorbent assay (ELISA).Results:There was a significant correlation between TLR4 mRNA and A20 mRNA in peripheral blood mononuclear cells(r =0.640,P =0.002).The expressions of A20 and TLR4 mRNA in monocytes in patients with ACS were higher than patients with SAP and healthy persons (P ＜ 0.001).Comparing with the single lesion group,TLR4 and A20 mRNA positive expression in the multivessel branch lesion group were increased (P =0.006,P =0.000).The levels of both tissue factor and TFPI in patients with ACS were significantly higher than the group of SAP and controls (P ＜0.01).Plasma tissue factor was statistically significant between the multivessel disease group and the single lesion group (P =0.017).The expression of TLR4 in PBMC group was positively related with the level of TF in ACS(r =0.718,P =0.000).Conclusion:The increased expression of TLR4 in monocytes was related to atherosclerotic plaque instability as well as coronary artery lesion degree.TLR4-mediated inflammatory pathway activation may promote clotting abnormalities and coronary artery thrombosis in patients with ACS.关键词
急性冠脉综合征/Toll样受体4/锌指蛋白A20/组织因子/组织因子途径抑制物Key words
acute coronary syndrome / toll-like receptor 4 / zinc finger protein A20 / tissue factor/ tissue factor passway inhibitor分类
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于春杨,王昭军,蒲肖君,陈小节..急性冠脉综合征外周血单核细胞Toll样受体4、锌指蛋白A20与组织因子的表达及意义[J].江苏大学学报（医学版）,2014,24(1):63-67,71,6.
