国际医药卫生导报2014，Vol.20Issue(6)：768-770,3.DOI:10.3760/cma.j.issn.1007-1245.2014.06.010
不同浓度的葡萄糖对INS-1细胞cAMP生成与胰岛素分泌的影响
Effect of different glucose concentrations on cAMP generation and insulin secretion in INS-1 cell
陈立强 1王洋洋 1司艳辉 2梁洁玲 1李海珠 1林德健 3李刚4
作者信息
- 1. 526021 肇庆市第一人民医院检验科
- 2. 526021 肇庆市第一人民医院血液内科
- 3. 526040 肇庆医学高等专科学校
- 4. 510663广州,广东吉赛生物科技有限公司
- 折叠
摘要
Abstract
Objective To analyze the effect of glucose on cAMP generation and insulin secretion in INS-1 cell.Method We detected the changes of cAMP and insulin in INS-1 cell at 30 min stimulation by glucose of different concentrations (2.8,7.5,15 mmol/L).Result cAMP generation and insulin secretion in INS-1 cell were obviously stimulated as glucose concentration increased (P ＜ 0.05) and were relative to the concentration of glucose.Conclusion The increasing concentration of glucose could induce the cAMP production and insulin secretion in INS-1 cell.关键词
葡萄糖/环磷酸腺苷/酶联免疫吸附试验/第二信使/胰岛素分泌Key words
Glucose/ cAMP/ ELISA/ Second messengers/ Insulin secretion引用本文复制引用
陈立强,王洋洋,司艳辉,梁洁玲,李海珠,林德健,李刚..不同浓度的葡萄糖对INS-1细胞cAMP生成与胰岛素分泌的影响[J].国际医药卫生导报,2014,20(6):768-770,3.
