中国病理生理杂志2014，Vol.30Issue(3)：394-399,6.DOI:10.3969/j.issn.1000-4718.2014.03.002
转录因子 YY1对口腔鳞癌细胞ITGB6基因表达的影响
Role of transcription factor YY1 in regulation of ITGB6 gene expression in oral squamous cell carcinoma
摘要
Abstract
AIM:To explore the role of transcription factor YY1 in the regulation of integrin β6 (ITGB6) gene expression in oral squamous cell carcinoma .METHODS: The distribution of the potential YY1 binding sites in ITGB6 promoter were predicted by bioinformatic methods .Series of 5′deletion of ITGB6 promoter luciferase reporter constructs containing potential YY1 binding sites were made and transfected into 293T cell line to detect the promoter activity .The binding activity of the transcription factor YY1 to ITGB6 promoter in the native chromatin environment was determined by chromatin immunoprecipitation (ChIP) assay.The role of the potential YY1 binding sites in the regulation of ITGB6 pro-moter activity was analyzed by substitution mutant analysis .The effect of YY1 over-expression on the mRNA expression of ITGB6 in oral squamous cell carcinoma cell line was measured by RT-PCR.RESULTS:Bioinformatics analysis revealed that there were several potential binding sites for YY 1 in the region of -421~-150 nt in the ITGB6 promoter.ChIP assay showed that transcription factor YY1 bound to the ITGB6 promoter region of -421~-150 nt in the native chromatin environ-ment.Substitution mutant analysis of potential YY 1 binding sites in ITGB6 promoter did not affect the promoter activity of ITGB6.The over-expression of YY1 in oral squamous cell carcinoma cells did not affect the ITGB 6 mRNA expression. CONCLUSION:The transcription factor YY1 binds to the region of -421~-150 nt in the ITGB6 promoter.However, it is not involved in the basic transcriptional regulation of ITGB6 gene in oral squamous cell carcinoma cells .关键词
YY1转录因子/整合素β6/口腔肿瘤Key words
YY1 transcription factor/Integrin β6/Mouth neoplasms分类
医药卫生引用本文复制引用
尹丽琴,许铭炎,陈锡和,舒申友,傅玉才,邓小玲..转录因子 YY1对口腔鳞癌细胞ITGB6基因表达的影响[J].中国病理生理杂志,2014,30(3):394-399,6.基金项目
国家自然科学基金资助项目(No.30900661 （）
No.81072208 （）
No.81370160) （）
教育部留学回国人员科研启动基金资助项目 （）
汕头大学医学院基础与临床科研基金资助项目 （）
