中国病理生理杂志2014，Vol.30Issue(3)：414-418,5.DOI:10.3969/j.issn.1000-4718.2014.03.006
一氧化氮／诱导型一氧化氮合酶在动脉粥样硬化过程中的作用
Effects of nitric oxide and inducible nitric oxide synthase on process of atherosclerosis
摘要
Abstract
AIM:To explore the dynamic changes of nitric oxide and inducible nitric oxide synthase during the process of atherosclerosis, and to analyze their influence on the formation of atherosclerosis .METHODS: SD rats (n=60) were randomly divided into control group and atherosclerosis group (30 rats in each group).Atherosclerosis model was induced by feeding high-fat diet and vitamin D 3 .The values of blood biochemical were analyzed enzymatically using bioMérieux kit.The concentration of serum nitric oxideing was detected by a colorimetric method .The success of athero-sclerosis modeling was determined by pathological examination .The protein expression of inducible nitric oxide synthase in atherosclerotic plaque was detected by the method of immunohistochemistry .RESULTS: The atherosclerosis model was successfully established in 90 d.The concentration of serum nitric oxide gradually decreased in atherosclerosis group , and a significant difference among groups was observed .Atherosclerosis index was positively correlated with calcium ion , and negatively correlated with nitric oxide .The protein expression of inducible nitric oxide synthase in the atherosclerotic plaque after 90 d was found.CONCLUSION:The protein expression of inducible nitric oxide synthase in the plaque area of aorta increases and the concentration of serum nitric oxide decreases with the process of atherosclerosis .The anti-atherosclerosis role of nitric oxide is gradually decreased .关键词
动脉粥样硬化/一氧化氮/一氧化氮合酶/钙Key words
Atherosclerosis/Nitric oxide/Nitric oxide synthase/Calcium分类
医药卫生引用本文复制引用
边宁,龚博君,郭军,黄正一,李自成..一氧化氮／诱导型一氧化氮合酶在动脉粥样硬化过程中的作用[J].中国病理生理杂志,2014,30(3):414-418,5.基金项目
国家自然科学基金资助项目(No.81371600) （No.81371600）
山西医科大学科技创新基金资助项目(No.057279) （No.057279）
山西医科大学博士启动基金资助项目(No.055217) （No.055217）
