中国病理生理杂志2014，Vol.30Issue(3)：423-429,7.DOI:10.3969/j.issn.1000-4718.2014.03.008
柚皮苷通过抑制瘦素通路对抗高糖引起的心肌细胞损伤
Naringin protects against high glucose-induced injury by inhibiting leptin pathway in H9c2 cardiac cells
摘要
Abstract
AIM: To study whether naringin protects H9c2 cardiac cells against high glucose (HG)-induced injury by inhibiting the leptin pathway .METHODS:The expression levels of leptin and leptin receptor (LEPR) were de-tected by Western blotting.The cell viability was analyzed by CCK-8 assay.The changes of the morphology and the number of apoptotic cells were tested by Hoechst 33258 nuclear staining.The intracellular levels of reactive oxygen species (ROS) were measured by DCFH-DA staining.Mitochondrial membrane potential (MMP) was determined by rhodamine 123 stai-ning.RESULTS:Treatment of the cells with 35 mmol/L glucose (HG) for 6~24 h up-regulated the expression of leptin in H9c2 cardiac cells with the peak value at 9 h.Treatment of the cells with HG for 1~24 h also enhanced the expression of LEPR, peaking at 12 h.Pretreatment with 80 μmol/L naringin for 2 h before exposure of the H9c2 cardiac cells to HG significantly inhibited the up-regulation of both leptin and LEPR induced by HG .Pretreatment of the cells with naringin for 2 h, leptin antagonist for 24 h, or leptin receptor antagonist for 2 h attenuated HG-induced injury in the cardiomyocytes , evidenced by an increase in cell viability , decreases in the number of apoptotic cells and intracellular ROS production as well as a recovery of MMP.CONCLUSION: Naringin may protect the cardiomyocytes against the HG-induced injury by inhibition of the leptin pathway.关键词
柚皮苷/瘦素/受体,瘦素/高血糖/心肌细胞Key words
Naringin/Leptin/Receptors, leptin/Hyperglycemia/Cardiomyocytes分类
医药卫生引用本文复制引用
刘付贞,陈景福,潘德茂,田丽红,郭润民,游琼,吴铿..柚皮苷通过抑制瘦素通路对抗高糖引起的心肌细胞损伤[J].中国病理生理杂志,2014,30(3):423-429,7.基金项目
安徽省国际合作计划项目(No.09080703042) （No.09080703042）
