中国病理生理杂志2014，Vol.30Issue(3)：460-466,7.DOI:10.3969/j.issn.1000-4718.2014.03.014
rhTGF-β1对 SD 大鼠 MSCs 骨向分化的影响及机制研究
Effects of rhTGF-β1 on proliferation and osteogenic differentiation of MSCs in SD rats
摘要
Abstract
AIM:To investigate the effects of recombinant human transforming growth factor β1 (rhTGF-β1 ) on the ability of proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells (MSCs), as well as its effects on the expression of bone morphogenetic protein 2 (BMP-2), Smad4 and core binding factor α1 (Cbfa1). METHODS:SD rat MSCs were isolated and purified by the differential time adherent method .MTT assay was used to con-firm the optimal concentration of rhTGF-β1 for the proliferation of MSCs.The optimal concentration for differentiation of MSCs into osteoblast was also determined by observing the activity and positive staining of alkaline phosphatase .According to the different induction conditions , MSCs were divided into 4 groups:control group, classic group, rhTGF-β1 group, and rhTGF-β1 +classic group.Alkaline phosphatase, type I collagen, bone Gla protein and calcium nodes were detected to e-valuate the osteogenic differentiation .BMP-2 was detected by ELISA and the mRNA expression of Smad4 and Cbfa1 was analyzed by real-time quantitative PCR.RESULTS:The optimal concentrations of rhTGF-β1 for the proliferation of MSCs and for the osteogenic differentiation of MSCs were 10 and 5μg/L, respectively.The MSCs in classical group and rhTGF-β1 group were promoted to osteogenic differentiation , and the mRNA expression of BMP-2, Smad4 and Cbfa1 was in-creased.rhTGF-β1 induced osteogenic differentiation of MSCs in the early and middle terms .However, in rhTGF-β1 +classic group, the osteogenic differentiation of MSCs was more obvious in the late term .CONCLUSION: The induction conditions of classical group , rhTGF-β1 group and rhTGF-β1 +classical group promote the differentiation of MSCs by in-creasing BMP-2 secretion and starting the TGF-βsuperfamily/Smads signaling pathway to regulate the differentiation of MSCs.关键词
骨髓间充质干细胞/成骨分化/转化生长因子Key words
Bone marrow mesenchymal stem cells/Osteogenic differentiation/Transforming growth factors分类
医药卫生引用本文复制引用
尹素娟,张荣华,朱晓峰,王攀攀,杨丽..rhTGF-β1对 SD 大鼠 MSCs 骨向分化的影响及机制研究[J].中国病理生理杂志,2014,30(3):460-466,7.基金项目
国家自然科学基金资助项目(No.81071114 （）
No.81371385) （）
