中国病理生理杂志2014，Vol.30Issue(3)：479-485,7.DOI:10.3969/j.issn.1000-4718.2014.03.017
人参皂苷 Rg1抑制叔丁基过氧化氢诱导的原代大鼠皮层神经元损伤
Ginsenoside Rg1 protects primary rat cerebrocortical neurons against t-BHP-induced damage in vitro
摘要
Abstract
AIM:To observe the effect of ginsenoside Rg1 (G-Rg1) on tert-butyl hydroperioxideo (t-BHP)-induced injury in primary rat cerebrocortical neurons and their filaments .METHODS:Primary rat cerebrocortical neurons were randomly divided into normal group , 10 μmol/L t-BHP induction group and 10 μmol/L t-BHP+10 μmol/L G-Rg1 treatment group.The MTT assay was used to detect the cell viability under various concentrations of t -BHP.3D cell recon-struction was performed to measure the filament length and number .The protein expression levels of Bcl-2, phosphorylated glycogen synthase kinase 3β(pGSK-3β) and caspase-3 were determined by the methods of immunofluorescence and West-ern blotting.RESULTS:G-Rg1 at concentration of 10 μmol/L reversed the decrease in cell viability , increased the pro-tein expression level of Bcl-2 and pGSK-3β, and suppressed the activation of caspase-3 in t-BHP induction group (P<0.05).CONCLUSION:G-Rg1 increases the anti-stress ability of the neurons by increasing the pGSK-3βphosphoylation under the condition of t-BHP exposure.关键词
人参皂苷Rg1/叔丁基过氧化氢/糖原合成酶激酶3βKey words
Ginsenoside Rg1/Tert-butyl hydroperioxide/Glycogen synthase kinase 3β分类
医药卫生引用本文复制引用
逯丹,舒晓明,张婵娟,赵佳仪,朱丽红,戚仁斌,王华东,陆大祥..人参皂苷 Rg1抑制叔丁基过氧化氢诱导的原代大鼠皮层神经元损伤[J].中国病理生理杂志,2014,30(3):479-485,7.基金项目
河北省卫生厅课题 (No.08282) （No.08282）
