中国病理生理杂志2014，Vol.30Issue(3)：524-528,5.DOI:10.3969/j.issn.1000-4718.2014.03.024
柯萨奇病毒 B3感染 HeLa 细胞后 Bax 和 Bim 的表达
Expression of Bax and Bim in coxsackievirus B3-infected HeLa cells
摘要
Abstract
AIM:To observe the expression of Bax and Bim at mRNA and protein levels in coxsackievirus B 3 (CVB3)-infected HeLa cells.METHODS:HeLa cells were divided into control (non-CVB3 infection) group and CVB3 infection group.The expression of Bim and Bax at mRNA and protein levels was determined by reverse transcriptase poly -merase chain reaction (RT-PCR) and Western blotting analysis at 3 h, 6 h, 9 h, 12 h and 24 h postinfection.RE-SULTS:The mRNA expression of Bax and Bim in control group was not significantly different among different time points , so was that in CVB3 infection group (all P>0.05).However, compared with control group, the mRNA expression of Bax and Bim in CVB3 infection groups was decreased at 24 h postinfection (all P<0.05).The protein expression of Bax and Bim in control group was not significantly different among different time points (P>0.05).However, in CVB3 infection group, the protein expression of Bax was lower at 24 h postinfection than that at 3 h (P<0.05).Meanwhile, compared with control group, the protein expression of Bax in CVB3 infection group was decreased at 24 h postinfection (P<0.05). The protein expression of Bim in CVB3 infection groups was decreased along with the time postinfection , and was decreased at 9 h, 12 h and 24 h as compared with control group (P<0.05).CONCLUSION:The proteins of Bax and Bim play an important role in CVB3-induced apoptosis of HeLa cells.关键词
HeLa细胞/柯萨奇病毒B3/细胞凋亡/Bax蛋白/Bim蛋白Key words
HeLa cells/Coxsackievirus B3/Apoptosis/Bax protein/Bim protein分类
医药卫生引用本文复制引用
杨丽,李欣,田朗,黄利华,李卓颖,杨作成..柯萨奇病毒 B3感染 HeLa 细胞后 Bax 和 Bim 的表达[J].中国病理生理杂志,2014,30(3):524-528,5.基金项目
四川省教育厅科研项目(No.11ZB223) （No.11ZB223）
