中国病理生理杂志2014，Vol.30Issue(3)：547-550,4.DOI:10.3969/j.issn.1000-4718.2014.03.029
膜联蛋白 A2对人宫颈癌 HeLa 细胞增殖、迁移和凋亡的影响
Effect of annexin A2 on proliferation, migration and apoptosis of human cervical cancer HeLa cells
摘要
Abstract
AIM:To explore the effect of annexin A2 (ANXA2) on the proliferation, migration and apoptosis abilities of human cervical cancer HeLa cells .METHODS: Overexpression vectors and siRNA of ANXA2 were construc-ted, and then transfected into HeLa cells .The HeLa cells were divided into 4 groups: control group, scramble group, ANXA2 overexpression group and ANXA2-siRNA group.The expression of ANXA2 at mRNA and protein levels was exam-ined by real-time PCR and Western blotting.MTT assay, Boyden chamber and flow cytometry were used to determine the effect of ANXA2 on the proliferation, migration and apoptosis abilities of the HeLa cells .RESULTS:The proliferation and migration of HeLa cells were obviously promoted by ANXA 2 overexpression.The proliferation and migration of HeLa cells were remarkably inhibited by the transfection of ANXA2-siRNA.ANXA2 had no effect on apoptosis of HeLa cells .CON-CLUSION:Silencing of ANXA2 effectively inhibits the proliferation and migration of cervical cancer cells , but has little effect on apoptosis.ANXA2 may play a pivotal role in the occurrence and development of cervical cancer , and may be used as a molecular target for the treatment of cervical cancer .关键词
膜联蛋白A2/HeLa细胞/细胞增殖/细胞迁移/细胞凋亡Key words
Annexin A2/HeLa cells/Cell proliferation/Cell migration/Apoptosis分类
医药卫生引用本文复制引用
魏建勋,马文君,李红梅..膜联蛋白 A2对人宫颈癌 HeLa 细胞增殖、迁移和凋亡的影响[J].中国病理生理杂志,2014,30(3):547-550,4.基金项目
国家自然科学基金资助项目(No.81171660) （No.81171660）
宁波市自然科学基金资助项目(No.2012A610207) （No.2012A610207）
宁波市科技创新团队资助项目(No.2011B82014) （No.2011B82014）
宁波市重点学科资助项目(No.XKL11D2127 （）
No.XKL11D2128) （）
宁波大学大学生科研创新计划资助项目(No.2013506) （No.2013506）
宁波大学优秀学位论文培育基金资助项目(No.PY2012004) （No.PY2012004）
