江苏大学学报（医学版）2014，Vol.24Issue(2)：110-113,4.DOI:10.13312/j.issn.1671-7783.y130262
小鼠血管平滑肌细胞原代培养及体外钙化模型的制备
A modified method for primary culture and calcification of aortic vascular smooth muscle cell of mouse
摘要
Abstract
Objective:To establish a simple and efficient method for primary culture of mouse aortic vascular smooth muscle cells (VSMCs) and calcification model in vitro.Methods:The primary VSMCs were harvested by modified tissue-piece inoculation and identified by immunocytochemistry.The established VSMCs were randomly divided into control group and calcification group.Calcification of cells was assayed by von kossa staining and colorimetry method.Results:VSMCs migrated from explants of mouse aorta tissue after 3-5 days of culture.After 7-10 days,VSMCs grew to form a fusing monolayer.Immunofluorescence staining with specific mAb against mouse α-actin demonstrated VSMCs were positive.The cell purity of the 2nd generation of VSMCs was over 95％.Compared with the control group,the calcium content and ALP activity in the calcification group were increased significantly (both P ＜ 0.05).Conclusion:The method of modified explant-culture successfully established a effective model for primary culture of VSMCs,of which calcification in vitro could be induced by β-glycerophosphate.关键词
血管平滑肌细胞/原代培养/钙化Key words
vascular smooth muscle cell/ primary culture/ calcification分类
医药卫生引用本文复制引用
徐丽华,严金川,伍超,逯朝阳,王中群,袁伟..小鼠血管平滑肌细胞原代培养及体外钙化模型的制备[J].江苏大学学报（医学版）,2014,24(2):110-113,4.基金项目
国家自然科学基金资助项目(81170279,81370409) （81170279,81370409）
江苏省自然基金及创新团队基金资助项目(BK2011486,LJ201116) （BK2011486,LJ201116）
镇江市心血管病重点实验室(SS2012002) （SS2012002）
