江苏大学学报（医学版）2014，Vol.24Issue(2)：126-128,3.DOI:10.13312/j.issn.1671-7783.y130281
HPLC法同时测定金针菇中麦角甾醇和22,23-二氢麦角甾醇的含量
High performance liquid chromatography for simultaneous determination of ergosterol and 22,23-dihydroergosterol in Flammulina velutipes
摘要
Abstract
Objective:To develop an efficient high performance liquid chromatography (HPLC) method for the simultaneous determination of ergosterol and 22,23-dihydroergosterol in Flammulina velutipes.Methods:Chromatographic separation was achieved on an Inertsil ODS-SP (250 mm × 4.6 mm,5 μm) analytical column using a mobile phase of 100％ methanol at 1.0 mL · min-1 The sterol content was detected at 282 nm.The temperature of column was 25 ℃.Results:The established linearity range for ergosterol and 22,23-dihydroergosterol was 0.025-1.5 μg (r =0.999 9).The average recoveries (n =6) were 104.6％ with RSD 0.89％,103.5％ with RSD 3.34％,respectively.Conclusion:The established HPLC method was simple,rapid and reliable for the separation and simultaneous determination of ergosterol and 22,23-dihydroergosterol in Flammulina velutipes.关键词
金针菇/麦角甾醇/22,23-二氢麦角甾醇/高效液相色谱法Key words
Flammulina velutipes/ ergosterol/ 22,23-dihydroergosterol/ high performance liquid chromatography分类
医药卫生引用本文复制引用
易承学,童珊珊,徐希明,余江南..HPLC法同时测定金针菇中麦角甾醇和22,23-二氢麦角甾醇的含量[J].江苏大学学报（医学版）,2014,24(2):126-128,3.基金项目
国家自然科学基金资助项目(30973677) （30973677）
教育部博士点基金资助项目(20113227110012) （20113227110012）
