癌变·畸变·突变Issue(6)：426-429,434,5.DOI:10.3969/j.issn.1004-616x.2013.06.005
静磁场对体外三维培养小鼠腔前卵泡发育成熟的影响
Effects of static magnetic field on proliferation of mouse preantral follicles 3D cultured in vitro
摘要
Abstract
OBJECTIVE: The reproductive toxicity of static magnetic field (SMF) was investigated using mouse preantral follicle 3D culture in vitro system established in our laboratory. METHODS:Ovaries was removed from 12 days old female mice. (130±10)μm preantral follicles were isolated and encapsulated into alginate hydrogel,then cultured in droplets for 8 days. Experimental groups were treated by different intensity (5 and 450 mT) SMF. Diameter of follicles was measured every other day. The ovulation of antral follicles was induced by HCG hormone,and the matured oocytes (first polar body emission) were identified under the stereomicroscope. RESULTS:Follicles in each group were well developed on morphologically. Follicular diameter of each group gradually increased with culture time. Follicular diameter of 450 mT SMF-treated group was significantly increased at 4 days (P<0.05),and diameter of 5 and 450 mT SMF-treated group was significantly increased at 6 days(P<0.05),compared with that of control group. With the increasing intensity of SMF,the cavity formation rate of the follicles was obviously elevated,but the oocytes maturation rate of 450 mT SMF-treated group was significantly reduced compared with that of the control group. CONCLUSION:The alginate hydrogel could maintains the 3D structure of individual mouse follicle. With increasing strength of SMF,the diameter of secondary follicles was statistically increased,the cavity formation ratio was also markedly increased,but the process of oocyte maturation was inhibited by 450 mT SMF.关键词
腔前卵泡/三维培养/卵子成熟/静磁场Key words
preantral follicle/3D culture/oocyte maturation/static magnetic field分类
医药卫生引用本文复制引用
林桂淼,马汉武,李媛,路滟,秦彦珉,易婉娴,梅树江,林苏霞,许改霞,李慧,朱玥荃,林晓潭,蔡志明,王晓梅..静磁场对体外三维培养小鼠腔前卵泡发育成熟的影响[J].癌变·畸变·突变,2013,(6):426-429,434,5.基金项目
广东省自然科学基金项目(S2012020001223)；深圳市科技计划项目(JC201005280391A)；深圳大学自然科学基金项目(201147) （S2012020001223）
